Protocols shared by authors via RaP service
Journal
Magnetic resonance imaging
Authors: Ellen Brenner and Martin Röcken
Date: Jul 14, 2021，Views：823
Original protocol used in research article：
Cancer immune control needs senescence induction by interferon-dependent cell cycle regulator pathways in tumours
Nature Communications, Mar 12, 2020; DOI: 10.1038/s41467-020-14987-6
Isolation of Extracellular Vesicles
Authors: Ciarra Almeria and Dominik Egger
Date: Jul 13, 2021，Views：839
Original protocol used in research article：
Hypoxia Conditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Induce Increased Vascular Tube Formation in vitro
Frontiers in Bioengineering and Biotechnology, Oct 23, 2019; DOI: 10.3389/fbioe.2019.00292
2.8. Cellular senescence quantitative assay
Author: Songying Zhang
Date: Jul 11, 2021，Views：962
Original protocol used in research article：
Excessive oxidative stress in cumulus granulosa cells induced cell senescence contributes to endometriosis-associated infertility
Redox Biology, Jan 12, 2020; DOI: 10.1016/j.redox.2020.101431
Quantification of ACE
Authors: Lishun Wang and Jiong Deng
Date: Jul 9, 2021，Views：893
Original protocol used in research article：
ALDH2 Repression Promotes Lung Tumor Progression via Accumulated Acetaldehyde and DNA Damage1
Neoplasia (New York, N.Y.), May 6, 2019; DOI: 10.1016/j.neo.2019.03.008
Mechanical stress assay
Author: Peng Huang
Date: Jul 9, 2021，Views：815
Original protocol used in research article：
Dual function of perivascular fibroblasts in vascular stabilization in zebrafish
PLoS Genetics, Oct 26, 2020; DOI: 10.1371/journal.pgen.1008800
qNano measurements
Author: Zoltán Wiener
Date: Jul 9, 2021，Views：820
Original protocol used in research article：
Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors
Cell Mol Life Sci, Apr 26, 2019; DOI: 10.1007/s00018-019-03052-1
ATAC-seq and ChIP-seq data analysis on mouse strains data
Authors: Zeyang Shen, Marten A Hoeksema and Christopher K. Glass
Date: Jul 8, 2021，Views：979
Original protocol used in research article：
Mechanisms underlying divergent responses of genetically distinct macrophages to IL-4
Science Advances, Jun 16, 2021; DOI: 10.1126/sciadv.abf9808
Protocol-BODIPY FL C5-Sphingomyelin or BODIPY FL C5-Ceramide staining in mESCs
Authors: Wei Fan and Xiaoling Li
Date: Jul 7, 2021，Views：1209
Original protocol used in research article：
SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B
eLife, May 27, 2021; DOI: 10.7554/eLife.67452
Spheroid-formation assay
Authors: Karen Legler and Leticia Oliveira-Ferrer
Date: Jul 7, 2021，Views：836
Original protocol used in research article：
Prognostic relevance of the Golgi mannosidase MAN1A1 in ovarian cancer: impact of N-glycosylation on tumour cell aggregation
British Journal of Cancer, Oct 29, 2019; DOI: 10.1038/s41416-019-0607-2
Cell-free degradation assays
Authors: Qingyan Shu and Liangsheng Wang
Date: Jul 7, 2021，Views：1452
Original protocol used in research article：
Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’
Journal of Experimental Botany, May 18, 2019; DOI: 10.1093/jxb/erz245
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