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Identification of signature genes of meta-clusters
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Jan 12, 2022，Views：861
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Data integration and meta-cluster identification
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Jan 12, 2022，Views：1009
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Phenotypic analysis of embryos
Authors: Eduardo D Gigante and Tamara Caspary
Date: Jan 12, 2022，Views：684
Original protocol used in research article：
ARL13B regulates Sonic hedgehog signaling from outside primary cilia
eLife, Mar 4, 2020; DOI: 10.7554/eLife.50434
Mathematical modeling of NF-κB
Authors: Minjun Son and Savaş Tay
Date: Jan 11, 2022，Views：731
Original protocol used in research article：
NF-κB responds to absolute differences in cytokine concentrations
Science Signaling, Jan 18, 2021; DOI: 10.1126/scisignal.aaz4382
Protein Production and Purification
Author: Nunziata Maio
Date: Jan 11, 2022，Views：699
Original protocol used in research article：
Fe-S cofactors in the SARS-CoV-2 RNA-dependent RNA polymerase are potential antiviral targets
Science, Jun 3, 2021; DOI: 10.1126/science.abi5224
Immunofluorescence and microscopy
Author: Alice H Huang
Date: Jan 11, 2022，Views：678
Original protocol used in research article：
Tgfβ signaling is required for tenocyte recruitment and functional neonatal tendon regeneration
eLife, Jun 5, 2020; DOI: 10.7554/eLife.51779
DNA templates and plasmids
Authors: Zachary F Mandell and Paul Babitzke
Date: Jan 11, 2022，Views：742
Original protocol used in research article：
NusG is an intrinsic transcription termination factor that stimulates motility and coordinates gene expression with NusA
eLife, Apr 9, 2021; DOI: 10.7554/eLife.61880
Adhesion scoring, whole-mount imaging, and tissue clearing
Authors: J. Zindel and P. Kubes
Date: Jan 11, 2022，Views：703
Original protocol used in research article：
Primordial GATA6 macrophages function as extravascular platelets in sterile injury
Science, Mar 4, 2021; DOI: 10.1126/science.abe0595
Quantitative RT-PCR
Authors: Yang Zhang and Huanghe Yang
Date: Jan 11, 2022，Views：680
Original protocol used in research article：
TMEM16F phospholipid scramblase mediates trophoblast fusion and placental development
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aba0310
Purification of murine B or T cells
Authors: Takuya Koike and Daisuke Kitamura
Date: Jan 6, 2022，Views：734
Original protocol used in research article：
The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets
eLife, Jun 21, 2019; DOI: 10.7554/eLife.44245
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