Protocols shared by authors via RaP service
Journal
Protocol of full article
Authors: Ryan Rahman and Richard H. Gomer
Date: Feb 23, 2022，Views：696
Original protocol used in research article：
Annotating Putative D. discoideum Proteins Using I-TASSER
microPublication Biology, ; DOI: 10.17912/micropub.biology.000420
Immunohistochemistry analysis
Authors: Hyuk-Kwon Kwon and Francis Y. Lee
Date: Feb 23, 2022，Views：655
Original protocol used in research article：
Dual therapeutic targeting of intra-articular inflammation and intracellular bacteria enhances chondroprotection in septic arthritis
Science Advances, Jun 24, 2021; DOI: 10.1126/sciadv.abf2665
Admixture-aware scan for signatures of local adaptation
Authors: Stephanie M Yan and Rajiv C McCoy
Date: Feb 23, 2022，Views：725
Original protocol used in research article：
Local adaptation and archaic introgression shape global diversity at human structural variant loci
eLife, Sep 16, 2021; DOI: 10.7554/eLife.67615
Study design
Authors: Thanaphong Phongpreecha and Thomas J. Montine
Date: Feb 23, 2022，Views：692
Original protocol used in research article：
Single-synapse analyses of Alzheimer’s disease implicate pathologic tau, DJ1, CD47, and ApoE
Science Advances, Dec 15, 2021; DOI: 10.1126/sciadv.abk0473
NMDA and light damage in mice
Author: Thanh Hoang
Date: Feb 21, 2022，Views：641
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
Single-cell RNA-seq (scRNA-seq) Methanol fixation
Author: Thanh Hoang
Date: Feb 21, 2022，Views：699
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
SMP isolation and culture in osteogenic media
Authors: Qian Cong and Yingzi Yang
Date: Feb 18, 2022，Views：700
Original protocol used in research article：
A self-amplifying loop of YAP and SHH drives formation and expansion of heterotopic ossification
Science Translational Medicine, Jun 23, 2021; DOI: 10.1126/scitranslmed.abb2233
Western blotting
Author: Akihiko Yokoyama
Date: Feb 13, 2022，Views：928
Original protocol used in research article：
HOXA9 promotes MYC-mediated leukemogenesis by maintaining gene expression for multiple anti-apoptotic pathways
eLife, Jul 26, 2021; DOI: 10.7554/eLife.64148
Histology
Authors: Nichola N Barron and David A Bechtold
Date: Feb 12, 2022，Views：981
Original protocol used in research article：
Adipocyte NR1D1 dictates adipose tissue expansion during obesity
eLife, Aug 5, 2021; DOI: 10.7554/eLife.63324
IIH6 Assays
Authors: Ameya Walimbe and Kevin P Campbell
Date: Feb 10, 2022，Views：844
Original protocol used in research article：
The functional O-mannose glycan on α-dystroglycan contains a phospho-ribitol primed for matriglycan addition
eLife, Apr 29, 2016; DOI: 10.7554/eLife.14473
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