Protocols shared by authors via RaP service
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Cell culture, transfection, and drug treatments
Authors: Jason E. Lee and Gia K. Voeltz
Date: Mar 2, 2022，Views：642
Original protocol used in research article：
Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI: 10.1126/science.aay7108
Cell activation and immunostaining
Author: Jesse Goyette
Date: Mar 2, 2022，Views：675
Original protocol used in research article：
Protein-PAINT: Superresolution microscopy with signaling proteins
Science Signaling, Feb 1, 2022; DOI: 10.1126/scisignal.abg9782
Conjunction of DNA and primary antibody
Authors: Xiaohao Liu, Fenyong Sun, Yu Yang, Xiaoli Zhu and Weihong Tan
Date: Mar 2, 2022，Views：722
Original protocol used in research article：
Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging
Science Advances, Jan 14, 2022; DOI: 10.1126/sciadv.abk0133
Islet dispersion
Author: J. E. Campbell
Date: Mar 2, 2022，Views：638
Original protocol used in research article：
GIP mediates the incretin effect and glucose tolerance by dual actions on α cells and β cells
Science Advances, Mar 12, 2021; DOI: 10.1126/sciadv.abf1948
Western blotting
Authors: Sthitapranjya Pati and Vidita A Vaidya
Date: Mar 1, 2022，Views：790
Original protocol used in research article：
Chronic postnatal chemogenetic activation of forebrain excitatory neurons evokes persistent changes in mood behavior
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56171
Microchannel migration imaging
Authors: Scott B Thompson and Jordan Jacobelli
Date: Feb 24, 2022，Views：685
Original protocol used in research article：
Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046
RNA interference
Authors: Shawn C. Chafe and Shoukat Dedhar
Date: Feb 24, 2022，Views：680
Original protocol used in research article：
Genome-wide synthetic lethal screen unveils novel CAIX-NFS1/xCT axis as a targetable vulnerability in hypoxic solid tumors
Science Advances, Aug 26, 2021; DOI: 10.1126/sciadv.abj0364
2.2. Nanoemulsion Preparation
Authors: Mandana Lotfi and Ali Akbar Moghadamnia
Date: Feb 23, 2022，Views：642
Original protocol used in research article：
Protective Effect of Quercetin Nanoemulsion on 5-Fluorouracil-Induced Oral Mucositis in Mice
Journal of Oncology, Jun 9, 2021; DOI: 10.1155/2021/5598230
Experimental tumor dormancy
Author: Masoud H. Manjili
Date: Feb 23, 2022，Views：633
Original protocol used in research article：
Autophagy-deficient breast cancer shows early tumor recurrence and escape from dormancy
Oncotarget, Apr 24, 2018; DOI: 10.18632/oncotarget.25197
Genome-wide association studies
Authors: Ella Katz and Daniel J Kliebenstein
Date: Feb 23, 2022，Views：732
Original protocol used in research article：
Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe
eLife, May 5, 2021; DOI: 10.7554/eLife.67784
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