Bio-101 - Improve Research Reproducibility

CD107a Degranulation Assay

Authors: Sareetha Kailayangiri and Claudia Rossig
Date: Jul 19, 2021，Views：1795

Original protocol used in research article：

EZH2 Inhibition in Ewing Sarcoma Upregulates GD2 Expression for Targeting with Gene-Modified T Cells
Molecular Therapy, Feb 23, 2019; DOI: 10.1016/j.ymthe.2019.02.014

Production and titration of SARS-CoV-2 spike pseudotyped virus using lentiviral system

Authors: Jie Hu, Qing-zhu Gao, Chang-long He, Ai-long Huang, Ni Tang and Kai Wang
Date: Jul 18, 2021，Views：824

Original protocol used in research article：

Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2
Genes & Diseases, Jul 17, 2020; DOI: 10.1016/j.gendis.2020.07.006

Linear copy and split–based whole-genome amplification

Author: Chenghang Zong
Date: Jul 16, 2021，Views：844

Original protocol used in research article：

Single-cell damagenome profiling unveils vulnerable genes and functional pathways in human genome toward DNA damage
Science Advances, Jul 1, 2021; DOI: 10.1126/sciadv.abf3329

Glycolysis capability (extracellular acidification) analysis.

Authors: Qian Shen, Heather M. Evans and Chad A. Rappleye
Date: Jul 16, 2021，Views：793

Original protocol used in research article：

Metabolism of Gluconeogenic Substrates by an Intracellular Fungal Pathogen Circumvents Nutritional Limitations within Macrophages
mBio, Apr 7, 2020; DOI: 10.1128/mBio.02712-19

2.4. Vascular permeability and Evans blue dye assay

Authors: Antonio Bernad and Diego Herrero
Date: Jul 16, 2021，Views：811

Original protocol used in research article：

Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart
Redox Biology, Mar 4, 2019; DOI: 10.1016/j.redox.2019.101156

GST fusion protein pulldown assays.

Authors: Insha Mushtaq, Vimla Band and Hamid Band
Date: Jul 15, 2021，Views：900

Original protocol used in research article：

EHD1 and RUSC2 Control Basal Epidermal Growth Factor Receptor Cell Surface Expression and Recycling
Molecular and Cellular Biology, Mar 16, 2020; DOI: 10.1128/MCB.00434-19

Cell corpse assays

Authors: Mor Levi-Ferber and Sivan Henis-Korenblit
Date: Jul 15, 2021，Views：770

Original protocol used in research article：

Transdifferentiation mediated tumor suppression by the endoplasmic reticulum stress sensor IRE-1 in C. elegans
eLife, Jul 20, 2015; DOI: 10.7554/eLife.08005

Microinjections into Zebrafish Embryos

Authors: Yuanyuan Sidi and Samuel Li
Date: Jul 14, 2021，Views：832

Original protocol used in research article：

An IRAK1-PIN1 Signalling Axis Drives Intrinsic Tumour Resistance to Radiation Therapy
Nat Cell Biol, Jan 21, 2019; DOI: 10.1038/s41556-018-0260-7

Transepithelial Electrical Resistance (TER) Measurements

Authors: Divya Sinha, Kimberly L. Edwards, Krishanu Saha and David M. Gamm
Date: Jul 14, 2021，Views：837

Original protocol used in research article：

Human iPSC Modeling Reveals Mutation-Specific Responses to Gene Therapy in a Genotypically Diverse Dominant Maculopathy
American Journal of Human Genetics, Jul 23, 2020; DOI: 10.1016/j.ajhg.2020.06.011

Quantification of carboxy-terminal fragment levels

Author: María-Paz Marzolo
Date: Jul 14, 2021，Views：788

Original protocol used in research article：

Participation of the SMAD2/3 signalling pathway in the down regulation of megalin/LRP2 by transforming growth factor beta (TGF-?1)
PLoS ONE, May 23, 2019; DOI: 10.1371/journal.pone.0213127

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