Protocols shared by authors via RaP service
Journal
Thioflavin S staining
Authors: Xiaoqian Peng and Weina Yang
Date: Dec 25, 2021，Views：798
Original protocol used in research article：
Tanshinone IIA attenuates neuroinflammation via inhibiting RAGE/NF-κB signaling pathway in vivo and in vitro
Journal of Neuroinflammation, Oct 14, 2020; DOI: 10.1186/s12974-020-01981-4
Measurement of vdark, vlight, and kd and estimation of kth
Authors: Keiichi Kojima and Takahiro Yamashita
Date: Dec 24, 2021，Views：678
Original protocol used in research article：
Evolutionary adaptation of visual pigments in geckos for their photic environment
Science Advances, Oct 1, 2021; DOI: 10.1126/sciadv.abj1316
Collection of xylem sap and phloem exudates
Authors: Zhen-Fei Chao and Dai-Yin Chao
Date: Dec 23, 2021，Views：918
Original protocol used in research article：
NPF transporters in synaptic-like vesicles control delivery of iron and copper to seeds
Science Advances, Sep 3, 2021; DOI: 10.1126/sciadv.abh2450
Assumptions on parameter values
Authors: Aurélien Marc and Jeremie Guedj
Date: Dec 23, 2021，Views：835
Original protocol used in research article：
Quantifying the relationship between SARS-CoV-2 viral load and infectiousness
eLife, Sep 27, 2021; DOI: 10.7554/eLife.69302
Chemical induction of hESC into goblet cells
Authors: Andong Zhao and Xiaobing Fu
Date: Dec 23, 2021，Views：701
Original protocol used in research article：
Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy
Science Advances, Apr 14, 2021; DOI: 10.1126/sciadv.abb2213
CCK-8 cell viability assay
Authors: Hui Che, Liping Wang and Yongxin Ren
Date: Dec 23, 2021，Views：1432
Original protocol used in research article：
p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52570
Quantifying cell proliferation through immunofluorescence on whole-mount and cryosectioned regenerating caudal fins in African killifish
Authors: Augusto Ortega Granillo, Robert Schnittker, Wei Wang and Alejandro Sánchez Alvarado
Date: Dec 20, 2021，Views：817
Original protocol used in research article：
Changes in regeneration-responsive enhancers shape regenerative capacities in vertebrates
Science, Sep 3, 2020; DOI: 10.1126/science.aaz3090
Molecular dynamics simulations of ASD-associated SHANK3 variants
Authors: Stephan Niebling, Michael Bucher and Marina Mikhaylova
Date: Dec 17, 2021，Views：1069
Original protocol used in research article：
Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses
eLife, May 4, 2021; DOI: 10.7554/eLife.66165
Cloning and expression of SARS-CoV-2 RBD in the yeast Pichia pastoris
Authors: Mirko Paiardini, Maria Elena Bottazzi and Sudhir Pai Kasturi
Date: Dec 15, 2021，Views：846
Original protocol used in research article：
A yeast expressed RBD-based SARS-CoV-2 vaccine formulated with 3M-052-alum adjuvant promotes protective efficacy in non-human primates
Science Immunology, Jul 15, 2021; DOI: 10.1126/sciimmunol.abh3634
Study design
Authors: Manoj Kumar, Souvik Maiti and Debojyoti Chakraborty
Date: Dec 9, 2021，Views：888
Original protocol used in research article：
FnCas9-based CRISPR diagnostic for rapid and accurate detection of major SARS-CoV-2 variants on a paper strip
eLife, Jun 9, 2021; DOI: 10.7554/eLife.67130
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