Protocols shared by authors via RaP service
Journal
Quantification of ACE
Authors: Lishun Wang and Jiong Deng
Date: Jul 9, 2021，Views：915
Original protocol used in research article：
ALDH2 Repression Promotes Lung Tumor Progression via Accumulated Acetaldehyde and DNA Damage1
Neoplasia (New York, N.Y.), May 6, 2019; DOI: 10.1016/j.neo.2019.03.008
Mechanical stress assay
Author: Peng Huang
Date: Jul 9, 2021，Views：836
Original protocol used in research article：
Dual function of perivascular fibroblasts in vascular stabilization in zebrafish
PLoS Genetics, Oct 26, 2020; DOI: 10.1371/journal.pgen.1008800
qNano measurements
Author: Zoltán Wiener
Date: Jul 9, 2021，Views：842
Original protocol used in research article：
Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors
Cell Mol Life Sci, Apr 26, 2019; DOI: 10.1007/s00018-019-03052-1
ATAC-seq and ChIP-seq data analysis on mouse strains data
Authors: Zeyang Shen, Marten A Hoeksema and Christopher K. Glass
Date: Jul 8, 2021，Views：1080
Original protocol used in research article：
Mechanisms underlying divergent responses of genetically distinct macrophages to IL-4
Science Advances, Jun 16, 2021; DOI: 10.1126/sciadv.abf9808
Protocol-BODIPY FL C5-Sphingomyelin or BODIPY FL C5-Ceramide staining in mESCs
Authors: Wei Fan and Xiaoling Li
Date: Jul 7, 2021，Views：1251
Original protocol used in research article：
SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B
eLife, May 27, 2021; DOI: 10.7554/eLife.67452
Spheroid-formation assay
Authors: Karen Legler and Leticia Oliveira-Ferrer
Date: Jul 7, 2021，Views：860
Original protocol used in research article：
Prognostic relevance of the Golgi mannosidase MAN1A1 in ovarian cancer: impact of N-glycosylation on tumour cell aggregation
British Journal of Cancer, Oct 29, 2019; DOI: 10.1038/s41416-019-0607-2
Cell-free degradation assays
Authors: Qingyan Shu and Liangsheng Wang
Date: Jul 7, 2021，Views：1565
Original protocol used in research article：
Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’
Journal of Experimental Botany, May 18, 2019; DOI: 10.1093/jxb/erz245
Labile Zinc Measurement by Flow Cytometry
Authors: Aleksha Panwar and Guruprasad R. Medigeshi
Date: Jul 6, 2021，Views：850
Original protocol used in research article：
Zinc Chelation Specifically Inhibits Early Stages of Dengue Virus Replication by Activation of NF-κB and Induction of Antiviral Response in Epithelial Cells
Frontiers in Immunology, Oct 1, 2019; DOI: 10.3389/fimmu.2019.02347
Immunoassay analysis of poly(GP)
Authors: Nadja S. Andrade and Zane Zeier
Date: Jul 6, 2021，Views：794
Original protocol used in research article：
Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD
Molecular Neurodegeneration, Feb 24, 2020; DOI: 10.1186/s13024-020-00365-9
Mitochondrial pyruvate transport assay
Authors: Robinson Arce-Molina, L Felipe Barros and Alejandro San Martín
Date: Jul 6, 2021，Views：960
Original protocol used in research article：
A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier MPC
eLife, Mar 6, 2020; DOI: 10.7554/eLife.53917
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