Protocols shared by authors via RaP service
Journal
LRP10 CRISPR/Cas9-mediated knockout in HuTu 80 cells
Authors: Martyna Grochowska and Wim Mandemakers
Date: Mar 14, 2022，Views：757
Original protocol used in research article：
LRP10 interacts with SORL1 in the intracellular vesicle trafficking pathway in non-neuronal brain cells and localises to Lewy bodies in Parkinson’s disease and dementia with Lewy bodies
Acta Neuropathol, Apr 28, 2021; DOI: 10.1007/s00401-021-02313-3
Differential abundance analysis of proteomics data
Authors: Sven Schenk, Stephanie C Bannister, Florian Raible and Kristin Tessmar-Raible
Date: Mar 11, 2022，Views：1782
Original protocol used in research article：
Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase
eLife, Feb 15, 2019; DOI: 10.7554/eLife.41556
Antibiotic treatment and fecal microbiota transplantation
Authors: Ping Li, Jing Li and E-Hu Liu
Date: Mar 10, 2022，Views：870
Original protocol used in research article：
Citrus polymethoxyflavones attenuate metabolic syndrome by regulating gut microbiome and amino acid metabolism
Science Advances, Jan 3, 2020; DOI: 10.1126/sciadv.aax6208
Isolation of lymphocytes from mouse skin
Authors: Joshua M. Moreau and Michael D. Rosenblum
Date: Mar 10, 2022，Views：1293
Original protocol used in research article：
Regulatory T cells promote innate inflammation after skin barrier breach via TGF-β activation
Science Immunology, Aug 26, 2021; DOI: 10.1126/sciimmunol.abg2329
Experiments
Author: Karen Alim
Date: Mar 9, 2022，Views：848
Original protocol used in research article：
Emergence of behaviour in a self-organized living matter network
eLife, Jan 21, 2022; DOI: 10.7554/eLife.62863
Reagents
Authors: Andrew J. Monteith and Eric P. Skaar
Date: Mar 9, 2022，Views：783
Original protocol used in research article：
Neutrophil extracellular traps enhance macrophage killing of bacterial pathogens
Science Advances, Sep 10, 2021; DOI: 10.1126/sciadv.abj2101
In vitro characterization of [3H]MK-6884 binding to brain tissues
Author: Wenping Li
Date: Mar 9, 2022，Views：688
Original protocol used in research article：
The PET tracer [11C]MK-6884 quantifies M4 muscarinic receptor in rhesus monkeys and patients with Alzheimer’s disease
Science Translational Medicine, Jan 12, 2022; DOI: 10.1126/scitranslmed.abg3684
Plant material
Author: P. R. RAMYA SREE
Date: Mar 9, 2022，Views：648
Original protocol used in research article：
Comparative Seed Morphology, Pharmacognostic, Phytochemical, and Antioxidant Potential of Memecylon L. Fruits
Turkish Journal of Pharmaceutical Sciences, Apr 20, 2021; DOI: 10.4274/tjps.galenos.2020.82956
Knockdown of FBN1 in vivo
Authors: Haiyan Fu and Youhua Liu
Date: Mar 9, 2022，Views：750
Original protocol used in research article：
Fibrillin-1–enriched microenvironment drives endothelial injury and vascular rarefaction in chronic kidney disease
Science Advances, Jan 27, 2021; DOI: 10.1126/sciadv.abc7170
Structural variants
Author: Kamil S. Jaron
Date: Mar 8, 2022，Views：696
Original protocol used in research article：
Convergent consequences of parthenogenesis on stick insect genomes
Science Advances, Feb 23, 2022; DOI: 10.1126/sciadv.abg3842
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