Protocols shared by authors via RaP service
Journal
3D nanoendoscopy-AFM
Authors: Marcos Penedo, Keisuke Miyazawa, Naoko Okano, Hirotoshi Furusho, Takehiko Ichikawa, Mohammad Shahidul Alam, Kazuki Miyata, Chikashi Nakamura and Takeshi Fukuma
Date: Jan 13, 2022，Views：695
Original protocol used in research article：
Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abj4990
Calculating the relative abundances of STs from metagenomic data
Authors: Jay-Hyun Jo and Heidi H. Kong
Date: Jan 12, 2022，Views：723
Original protocol used in research article：
Alterations of human skin microbiome and expansion of antimicrobial resistance after systemic antibiotics
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abd8077
scRNA-Seq data generation
Authors: Ranran Gao, Xueda Hu and Zemin Zhang
Date: Jan 12, 2022，Views：958
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
scRNA-Seq data preprocessing
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Jan 12, 2022，Views：1112
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Gene regulatory network analysis
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Jan 12, 2022，Views：968
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Identification of signature genes of meta-clusters
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Jan 12, 2022，Views：894
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Data integration and meta-cluster identification
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Jan 12, 2022，Views：1038
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Phenotypic analysis of embryos
Authors: Eduardo D Gigante and Tamara Caspary
Date: Jan 12, 2022，Views：722
Original protocol used in research article：
ARL13B regulates Sonic hedgehog signaling from outside primary cilia
eLife, Mar 4, 2020; DOI: 10.7554/eLife.50434
Mathematical modeling of NF-κB
Authors: Minjun Son and Savaş Tay
Date: Jan 11, 2022，Views：752
Original protocol used in research article：
NF-κB responds to absolute differences in cytokine concentrations
Science Signaling, Jan 18, 2021; DOI: 10.1126/scisignal.aaz4382
Protein Production and Purification
Author: Nunziata Maio
Date: Jan 11, 2022，Views：727
Original protocol used in research article：
Fe-S cofactors in the SARS-CoV-2 RNA-dependent RNA polymerase are potential antiviral targets
Science, Jun 3, 2021; DOI: 10.1126/science.abi5224
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