Protocols shared by authors via RaP service
Journal
Immunofluorescence on adherent cells using anti-phosphatidylinositol (PI) antibodies
Authors: Federico Gulluni and Emilio Hirsch
Date: Dec 29, 2021，Views：755
Original protocol used in research article：
PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation
Science, Dec 10, 2021; DOI: 10.1126/science.abk0410
Hypoxia, perfusion, and vascular leakage measurement
Authors: Yi Sun, Lieping Chen and Yuwen Zhu
Date: Dec 27, 2021，Views：749
Original protocol used in research article：
Blockade of the CD93 pathway normalizes tumor vasculature to facilitate drug delivery and immunotherapy
Science Translational Medicine, Jul 28, 2021; DOI: 10.1126/scitranslmed.abc8922
Thioflavin S staining
Authors: Xiaoqian Peng and Weina Yang
Date: Dec 25, 2021，Views：769
Original protocol used in research article：
Tanshinone IIA attenuates neuroinflammation via inhibiting RAGE/NF-κB signaling pathway in vivo and in vitro
Journal of Neuroinflammation, Oct 14, 2020; DOI: 10.1186/s12974-020-01981-4
Measurement of vdark, vlight, and kd and estimation of kth
Authors: Keiichi Kojima and Takahiro Yamashita
Date: Dec 24, 2021，Views：667
Original protocol used in research article：
Evolutionary adaptation of visual pigments in geckos for their photic environment
Science Advances, Oct 1, 2021; DOI: 10.1126/sciadv.abj1316
Collection of xylem sap and phloem exudates
Authors: Zhen-Fei Chao and Dai-Yin Chao
Date: Dec 23, 2021，Views：883
Original protocol used in research article：
NPF transporters in synaptic-like vesicles control delivery of iron and copper to seeds
Science Advances, Sep 3, 2021; DOI: 10.1126/sciadv.abh2450
Assumptions on parameter values
Authors: Aurélien Marc and Jeremie Guedj
Date: Dec 23, 2021，Views：811
Original protocol used in research article：
Quantifying the relationship between SARS-CoV-2 viral load and infectiousness
eLife, Sep 27, 2021; DOI: 10.7554/eLife.69302
Chemical induction of hESC into goblet cells
Authors: Andong Zhao and Xiaobing Fu
Date: Dec 23, 2021，Views：681
Original protocol used in research article：
Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy
Science Advances, Apr 14, 2021; DOI: 10.1126/sciadv.abb2213
CCK-8 cell viability assay
Authors: Hui Che, Liping Wang and Yongxin Ren
Date: Dec 23, 2021，Views：1375
Original protocol used in research article：
p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52570
Quantifying cell proliferation through immunofluorescence on whole-mount and cryosectioned regenerating caudal fins in African killifish
Authors: Augusto Ortega Granillo, Robert Schnittker, Wei Wang and Alejandro Sánchez Alvarado
Date: Dec 20, 2021，Views：799
Original protocol used in research article：
Changes in regeneration-responsive enhancers shape regenerative capacities in vertebrates
Science, Sep 3, 2020; DOI: 10.1126/science.aaz3090
Molecular dynamics simulations of ASD-associated SHANK3 variants
Authors: Stephan Niebling, Michael Bucher and Marina Mikhaylova
Date: Dec 17, 2021，Views：1040
Original protocol used in research article：
Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses
eLife, May 4, 2021; DOI: 10.7554/eLife.66165
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.