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Antibiotic treatment and fecal microbiota transplantation

PL Ping Li
JL Jing Li
EL E-Hu Liu

Last updated date: Mar 10, 2022 Views: 672 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Jan, 2020
Citrus polymethoxyflavones attenuate metabolic syndrome by regulating gut microbiome and amino acid metabolism
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Antibiotic treatment

Antibiotic administration in the drinking water of mice (vancomycin (0.5 g/liter), neomycin sulfate (1 g/liter), metronidazole (1 g/liter), and ampicillin (1 g/liter)) daily. 

 

 Fecal microbiota transplantation

Stool was collected daily from donor mice and pooled. Donor stool was diluted with saline and homogenized for 1 min using a vortex to achieve a liquid slurry (0.1 g/mL), and then centrifuged at 500g for 3 min to remove particulate matter to facilitate administration. Fresh transplant material (0.2mL/10g) was prepared within 10 min before oral gavage to prevent changes in bacterial composition.

 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Li, P, Li, J and Liu, E(2022). Antibiotic treatment and fecal microbiota transplantation. Bio-protocol Preprint. bio-protocol.org/prep1581.
  2. Zeng, S., Li, S., Xiao, P., Cai, Y., Chu, C., Chen, B., Li, P., Li, J. and Liu, E.(2020). Citrus polymethoxyflavones attenuate metabolic syndrome by regulating gut microbiome and amino acid metabolism. Science Advances 6(1). DOI: 10.1126/sciadv.aax6208

Category

Medicine

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