Proliferation assay and cell cycle profiling via flow cytometry

Proliferation assay

1. Plate proper number of cells into 6-well plates (represent Day 1 to Day 6
   respectively). For example, 30,000 BJ cells per well is optimal for 5-6 days of
   proliferation. Number of cell seeded should be modified to make sure the cells are
   not over grown at the last day of the experiment.
2. Perform treatment to the cells after they are well-adhered.
3. From the second day (Day 1), wash cells with PBS and trypsinize each well of cells
   per day. Use hemocytometer or automated cell counter (e.g. Cellometer^® K2
   Fluorescent Cell Counter of Nexcelom) to count cells every day, until the last day.
4. Attention should be paid that foam should be avoid when cells are resuspended,
   which would effects counting accuracy.
    

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