Protocols shared by authors via RaP service
Journal
2.2.2. Maceration Extraction (ME)
Authors: Jurga Andreja Kazlauskaite and Jurga Bernatoniene
Date: Mar 6, 2022，Views：607
Original protocol used in research article：
Novel Extraction Method Using Excipients to Enhance Yield of Genistein and Daidzein in Trifolium pratensis L.
Pharmaceutics, May 22, 2021; DOI: 10.3390/pharmaceutics13060777
Drug preparation
Author: Lu Chen
Date: Mar 6, 2022，Views：859
Original protocol used in research article：
Defective memory engram reactivation underlies impaired fear memory recall in Fragile X syndrome
eLife, Nov 20, 2020; DOI: 10.7554/eLife.61882
Phytaspase cleavage assay using synthetic oligopeptide substrates
Authors: A. Stintzi and A. Schaller
Date: Mar 4, 2022，Views：663
Original protocol used in research article：
Peptide signaling for drought-induced tomato flower drop
Science, Mar 26, 2020; DOI: 10.1126/science.aaz5641
Identification of individual potentially tumor-reactive T cells
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Mar 3, 2022，Views：797
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Indexes characterizing meta-clusters and STARTRAC analysis
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Mar 3, 2022，Views：947
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Immunohistochemistry
Authors: Jose Felix Moruno Manchon and Andrey S Tsvetkov
Date: Mar 3, 2022，Views：682
Original protocol used in research article：
Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons
eLife, Feb 11, 2020; DOI: 10.7554/eLife.52283
Cell cultures and transfection
Authors: Jose Felix Moruno Manchon and Andrey S Tsvetkov
Date: Mar 3, 2022，Views：689
Original protocol used in research article：
Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons
eLife, Feb 11, 2020; DOI: 10.7554/eLife.52283
DiI tracing
Authors: Nicolas Renier and Alain Chédotal
Date: Mar 3, 2022，Views：666
Original protocol used in research article：
A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex
eLife, Mar 28, 2017; DOI: 10.7554/eLife.23494
Selection of genetic instruments
Authors: Wuqing Huang, Jun Xiao, Jianguang Ji and Liangwan Chen
Date: Mar 2, 2022，Views：754
Original protocol used in research article：
Association of lipid-lowering drugs with COVID-19 outcomes from a Mendelian randomization study
eLife, Dec 6, 2021; DOI: 10.7554/eLife.73873
Microinjection and selection and confirmation of transgenic zebrafish
Author: Chiou-Hwa Yuh
Date: Mar 2, 2022，Views：601
Original protocol used in research article：
l-Carnitine ameliorates congenital myopathy in a tropomyosin 3 de novo mutation transgenic zebrafish
Journal of Biomedical Science, Jan 12, 2021; DOI: 10.1186/s12929-020-00707-1
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