Bio-101 - Improve Research Reproducibility

Primary osteoclast culture

Authors: Eben G Estell and Clifford J Rosen
Date: Mar 7, 2022，Views：856

Original protocol used in research article：

Irisin directly stimulates osteoclastogenesis and bone resorption in vitro and in vivo
eLife, Aug 11, 2020; DOI: 10.7554/eLife.58172

Larvae dissociation

Authors: Maria Ina Arnone and Periklis Paganos
Date: Mar 7, 2022，Views：650

Original protocol used in research article：

Single-cell RNA sequencing of the Strongylocentrotus purpuratus larva reveals the blueprint of major cell types and nervous system of a non-chordate deuterostome
eLife, Nov 25, 2021; DOI: 10.7554/eLife.70416

Animal models

Authors: I-Lin Ho and Andrea Viale
Date: Mar 7, 2022，Views：623

Original protocol used in research article：

Epithelial memory of inflammation limits tissue damage while promoting pancreatic tumorigenesis
Science, Sep 17, 2021; DOI: 10.1126/science.abj0486

Senescence-associated β-galactosidase assay

Authors: Mounir El Maï and Miguel Godinho Ferreira
Date: Mar 7, 2022，Views：841

Original protocol used in research article：

Opposing p53 and mTOR/AKT promote an in vivo switch from apoptosis to senescence upon telomere shortening in zebrafish
eLife, May 19, 2020; DOI: 10.7554/eLife.54935

Crown delineation

Author: Ben G Weinstein
Date: Mar 7, 2022，Views：658

Original protocol used in research article：

A remote sensing derived data set of 100 million individual tree crowns for the National Ecological Observatory Network
eLife, Feb 19, 2021; DOI: 10.7554/eLife.62922

2.2.2. Maceration Extraction (ME)

Authors: Jurga Andreja Kazlauskaite and Jurga Bernatoniene
Date: Mar 6, 2022，Views：577

Original protocol used in research article：

Novel Extraction Method Using Excipients to Enhance Yield of Genistein and Daidzein in Trifolium pratensis L.
Pharmaceutics, May 22, 2021; DOI: 10.3390/pharmaceutics13060777

Drug preparation

Author: Lu Chen
Date: Mar 6, 2022，Views：632

Original protocol used in research article：

Defective memory engram reactivation underlies impaired fear memory recall in Fragile X syndrome
eLife, Nov 20, 2020; DOI: 10.7554/eLife.61882

Phytaspase cleavage assay using synthetic oligopeptide substrates

Authors: A. Stintzi and A. Schaller
Date: Mar 4, 2022，Views：623

Original protocol used in research article：

Peptide signaling for drought-induced tomato flower drop
Science, Mar 26, 2020; DOI: 10.1126/science.aaz5641

Identification of individual potentially tumor-reactive T cells

Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Mar 3, 2022，Views：752

Original protocol used in research article：

Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474

Indexes characterizing meta-clusters and STARTRAC analysis

Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Mar 3, 2022，Views：905

Original protocol used in research article：

Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474

First
Prev

Next
Last

Protocols 

Request a Protocol

Receive Our Alerts

Submit 

About