Single-cell photoablation of MN2

Dear Stolfi,

This is the sequence.

LOCUS       pSP_NGN_minisog2        4297 bp ds-DNA     circular     21-OCT-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
 ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
    misc_feature    1444..1919
                    /locus_tag="SV40terminal"
                    /ApEinfo_fwdcolor="#c0c0c0"
                    /ApEinfo_revcolor="green"
                    /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                    width 5 offset 0"
    source          1057..1374
                    /organism="synthetic DNA construct"
                    /mol_type="other DNA"
                    /locus_tag="source:synthetic DNA construct"
                    /ApEinfo_fwdcolor="pink"
                    /ApEinfo_revcolor="pink"
                    /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                    width 5 offset 0"
    misc_feature    1057..1374
                    /locus_tag="minisog2"
                    /ApEinfo_fwdcolor="#ff0000"
                    /ApEinfo_revcolor="green"
                    /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                    width 5 offset 0"
    misc_feature    1375..1443
                    /locus_tag="CAAX"
                    /ApEinfo_fwdcolor="#ff8000"
                    /ApEinfo_revcolor="green"
                    /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                    width 5 offset 0"
    misc_feature    1507..1524
                    /locus_tag="primer_rev_seq"
                    /ApEinfo_fwdcolor="cyan"
                    /ApEinfo_revcolor="green"
                    /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                    width 5 offset 0"
    misc_feature    52..1037
                    /locus_tag="neurogenin promoter"
                    /ApEinfo_fwdcolor="#ff80ff"
                    /ApEinfo_revcolor="green"
                    /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                    width 5 offset 0"
    misc_feature    1021..1036
                    /locus_tag="primer_fwd_seq"
                    /ApEinfo_fwdcolor="cyan"
                    /ApEinfo_revcolor="green"
                    /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                    width 5 offset 0"
ORIGIN
       1 gaactcgagc agctgaagct tgcatgcCTG CAGGTCGACT CTAGAGGATC CGGAAGAGGT
      61 GTTAGANNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     121 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TACCCCAACN
     181 NATACTGTAT TTAAAGACGA CGTAAGTTGG CATGTAGTCG CATTTAAATT CTGAGCAATA
     241 TTTTTGNGTC TCTCGGCAGC GCGCATGAGG TTTCGGCGTA TAAGAGANCC AAGTGCACTT
     301 TCCCACAGAA GAAGCGGTGA ATATAATACA GACATAGTAA TGTCTGGTGC CGCAGCAAAA
     361 TNNNAGGTTC CCATGCATAT GTCCCCGTAT ACTATCGCCA GACAAGGTAA CTTTCCCACT
     421 CAGAAAAACG GTGTCCAACC GCGGTCATGC TTTGTTACGT CCCAGAGTCA AGGTCAGGTA
     481 TTGGCCCGGT CGTCTGTTCG GTTTTTGAGA TTCGTTTCCA CTCGGAAAAC GCTATTGTTT
     541 CATCGCTATC CATTTGTTTG CTAACGTCTA AGTCGCCGCC CGCATTTGCT GGAAGATTTG
     601 CTTTTTTCTC CGATTTGTGA CGTCACAGTG CTTCGGCTGA TCTCTCTGTT TCATCCCGAC
     661 GCGCCTTTTT CTCTACTTTC TCTCGGAGCT ATATAAGCAG ATCGTAATTT CTACTTTCAC
     721 TATAATCTGT TTTGGTTCTA CAGAGCCTAG TGGCTCATAT TTGCAGAATG CATTCGCAGC
     781 ATTTCTGGCA AATATTTCGT ATTAACAAAA CCGGCCAGGC CAGGGCTTTT TGCAAAAGGC
     841 ATTGTGACAT ATATTTTGTA TATGTCAAAC TCTAGCTAAA GTTTAAAGTT ATTCAAAACT
     901 ATTTTAGGAA AGCACGGAGA GCAGAATACC ATATTTTATT GCTAAAATTT CTTTTTCTTC
     961 AGAAACTTAA CAAATATCTT ATTTCATTTC AGATTTCAAT CTTGATATTA TAAAAATTTT
    1021 GCTCTTGCTA CAAAATAGGA TCCCCTTGCG GCCGCtatgg aaaaaagctt cgtgattact
    1081 gacccgcgcc tgccggataa tccgatcatc ttcgcctccg atagtttcct ggaactgact
    1141 gaatacagcc gtgaggaaat tctgggtcgt aatccccgtt tcctgcgggg cccggaaacc
    1201 gatcaagcaa ctgttcagaa aattcatgat gcaatccgtg atcaacgtga aatcaccgtg
    1261 cagctgatta actataccaa aagcggcaag aaattctgga acttgtttcg cctgcagccg
    1321 attcgcgatc agaaaggtga gctgcagtac ttcatcgggg ttcagctgga tggtGAATTC
    1381 AAGATGAGCA AGGACGGCAA GAAGAAAAAG AAGAAAAGCA AGACCAAGTG CGTGATCATG
    1441 TAAgaattcC AGCTGAGCGC CGGTCGCTAC CATTACCAGT TGGTCTGGTG TCAAAAATAA
    1501 TAATAACCGG GCAGGCCATG TCTGCCCGTA TTTCGCGTAA GGAAATCCAT TATGTACTAT
    1561 TTAAAAAACA CAAACTTTTG GATGTTCGGT TTATTCTTTT TCTTTTACTT TTTTATCATG
    1621 GGAGCCTACT TCCCGTTTTT CCCGATTTGG CTACATGACA TCAACCATAT CAGCAAAAGT
    1681 GATACGGGTA TTATTTTTGC CGCTATTTCT CTGTTCTCGC TATTATTCCA ACCGCTGTTT
    1741 GGTCTGCTTT CTGACAAACT CGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
    1801 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
    1861 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCGA CAAAGTCAAA GCGGCCATCA
    1921 GATCTGCcgg tctccctata gtgagtcgta ttaatttcga taagccaggt taacctgcat
    1981 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    2041 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    2101 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    2161 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    2221 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    2281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    2341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    2401 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    2461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    2521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    2581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    2641 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    2701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    2761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    2821 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    2881 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    2941 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    3001 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    3061 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    3121 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    3181 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    3241 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    3301 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    3361 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    3421 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    3481 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    3541 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    3601 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    3661 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    3721 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    3781 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    3841 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    3901 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    3961 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    4021 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    4081 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    4141 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
    4201 ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc
    4261 ttatgtatca tacacatacg atttaggtga cactata  
//