Bio-101 - Improve Research Reproducibility

Data Analysis

Authors: Tianying Zhong and Yun Gu
Date: Mar 27, 2022，Views：603

Original protocol used in research article：

Label-Free Quantitative Comparison of Cervical Mucus Peptides in Subjects With Endocervical Adenocarcinoma and Adenocarcinoma in Situ
Technology in Cancer Research & Treatment, Feb 22, 2021; DOI: 10.1177/1533033821997825

Slingshot

Authors: Bryan Marsh and Robert Blelloch
Date: Mar 24, 2022，Views：700

Original protocol used in research article：

Single nuclei RNA-seq of mouse placental labyrinth development
eLife, Nov 3, 2020; DOI: 10.7554/eLife.60266

Generation of E. coli strains

Authors: Shar-yin N. Huang, Nadim Majdalani and Yves Pommier
Date: Mar 22, 2022，Views：701

Original protocol used in research article：

Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes
Science Advances, Mar 3, 2021; DOI: 10.1126/sciadv.abe0384

Rotarod

Authors: Erik C. Böttger and David Paul Wolfer
Date: Mar 22, 2022，Views：678

Original protocol used in research article：

Premature aging in mice with error-prone protein synthesis
Science Advances, Mar 2, 2022; DOI: 10.1126/sciadv.abl9051

Ubiquitination assay

Authors: Di Feng and Bo Gao
Date: Mar 22, 2022，Views：3000

Original protocol used in research article：

Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7–mediated Vangl ubiquitination and endoplasmic reticulum–associated degradation
Science Advances, May 14, 2021; DOI: 10.1126/sciadv.abg2099

CRISPR knockout and siRNA knockdown

Authors: Zhiping Weng and Kevin Struhl
Date: Mar 22, 2022，Views：767

Original protocol used in research article：

YAP and TAZ are transcriptional co-activators of AP-1 proteins and STAT3 during breast cellular transformation
eLife, Aug 31, 2021; DOI: 10.7554/eLife.67312

Culture conditions

Authors: Milo S Johnson and Michael M Desai
Date: Mar 21, 2022，Views：744

Original protocol used in research article：

Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations
eLife, Jan 19, 2021; DOI: 10.7554/eLife.63910

Supervised classification

Authors: Diptendu Mukherjee, Eyal Itskovits and Ami Citri
Date: Mar 21, 2022，Views：660

Original protocol used in research article：

Salient experiences are represented by unique transcriptional signatures in the mouse brain
eLife, Feb 7, 2018; DOI: 10.7554/eLife.31220

Expression and preparation of proteins

Authors: Tao Xie, Tamjeed Saleh, Paolo Rossi and Charalampos G. Kalodimos
Date: Mar 21, 2022，Views：685

Original protocol used in research article：

Conformational states dynamically populated by a kinase determine its function
Science, Oct 8, 2020; DOI: 10.1126/science.abc2754

Preparation for two-photon calcium imaging in visual cortex

Authors: Carsen Stringer, Marius Pachitariu and Kenneth D. Harris
Date: Mar 18, 2022，Views：623

Original protocol used in research article：

Spontaneous behaviors drive multidimensional, brainwide activity
Science, Apr 19, 2019; DOI: 10.1126/science.aav7893

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