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Preprint

10x genomics

ST Sudhir Gopal Tattikota
NP Norbert Perrimon

Last updated date: May 27, 2022 Views: 647 Forks: 0

original An abbreviated version of this protocol was published in eLife in May, 2020
A single-cell survey of Drosophila blood
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For a detailed protocol for scRNA-seq using 10x pipeline, we followed exactly all the instructions as provided by the manufacturer. Please visit 10x portal for more details: https://support.10xgenomics.com/single-cell-gene-expression/library-prep/doc/user-guide-chromium-single-cell-3-reagent-kits-user-guide-v31-chemistry 

 

For a detailed protocol on Drosophila larval blood cell isolation for scRNA-seq, please see: Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing by Tattikota and Perrimon, BioProtocol, 2021  https://bio-protocol.org/e4127

 

 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Tattikota, S and Perrimon, N(2022). 10x genomics. Bio-protocol Preprint. bio-protocol.org/prep1689.
  2. Tattikota, S. G., Cho, B., Liu, Y., Hu, Y., Barrera, V., Steinbaugh, M. J., Yoon, S., Comjean, A., Li, F., Dervis, F., Hung, R., Nam, J., Ho Sui, S., Shim, J. and Perrimon, N.(2020). A single-cell survey of Drosophila blood. eLife. DOI: 10.7554/eLife.54818

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