Protocols shared by authors via RaP service
Journal
Dot blot assay
Authors: Yong-gui Wu and Xiao-ming Meng
Date: Apr 29, 2022，Views：813
Original protocol used in research article：
Inhibition of METTL3 attenuates renal injury and inflammation by alleviating TAB3 m6A modifications via IGF2BP2-dependent mechanisms
Science Translational Medicine, Apr 13, 2022; DOI: 10.1126/scitranslmed.abk2709
IMC data
Authors: James N Arnold and Shahram Kordasti
Date: Apr 28, 2022，Views：676
Original protocol used in research article：
ImmunoCluster provides a computational framework for the nonspecialist to profile high-dimensional cytometry data
eLife, Apr 30, 2021; DOI: 10.7554/eLife.62915
Isolation and cryopreservation of gonads
Authors: Michael J McGrew and Jun Hu
Date: Apr 28, 2022，Views：693
Original protocol used in research article：
A low-tech, cost-effective and efficient method for safeguarding genetic diversity by direct cryopreservation of poultry embryonic reproductive cells
eLife, Jan 25, 2022; DOI: 10.7554/eLife.74036
JAGuaR Alignment
Author: Richard Corbett
Date: Apr 26, 2022，Views：602
Original protocol used in research article：
A Scalable Strand-Specific Protocol Enabling Full-Length Total RNA Sequencing From Single Cells
Frontiers in Genetics, Jun 3, 2021; DOI: 10.3389/fgene.2021.665888
Preparation of bovine serum albumin (BSA)-conjugated palmitic acid
Author: Fei Hua
Date: Apr 26, 2022，Views：1092
Original protocol used in research article：
Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway
BMC Nephrology, Jun 10, 2021; DOI: 10.1186/s12882-021-02391-1
Proliferation assay and cell cycle profiling via flow cytometry
Author: Li Lan
Date: Apr 26, 2022，Views：646
Original protocol used in research article：
cGAS suppresses genomic instability as a decelerator of replication forks
Science Advances, Oct 13, 2020; DOI: 10.1126/sciadv.abb8941
Immunohistochemistry
Author: Sungjin Park
Date: Apr 25, 2022，Views：627
Original protocol used in research article：
The release of surface-anchored α-tectorin an apical extracellular matrix protein mediates tectorial membrane organization
Science Advances, Nov 26, 2019; DOI: 10.1126/sciadv.aay6300
Coevolutionary analysis and coarse-grained simulations
Authors: Francesco Colizzi and Modesto Orozco
Date: Apr 25, 2022，Views：645
Original protocol used in research article：
Probing allosteric regulations with coevolution-driven molecular simulations
Science Advances, Sep 8, 2021; DOI: 10.1126/sciadv.abj0786
Single-cell TCR sequencing
Authors: Daniel T. Leung and Owen Jensen
Date: Apr 22, 2022，Views：659
Original protocol used in research article：
A subset of follicular helper-like MAIT cells can provide B cell help and support antibody production in the mucosa
Science Immunology, Jan 14, 2022; DOI: 10.1126/sciimmunol.abe8931
mAb competition assay
Authors: Tomas Strandin, Andrew S. Herbert, Mattias N. E. Forsell, Laura M. Walker and Kartik Chandran
Date: Apr 21, 2022，Views：587
Original protocol used in research article：
Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
Science Translational Medicine, Mar 16, 2022; DOI: 10.1126/scitranslmed.abl5399
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