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Detection of SARS-CoV-2 specific IgG and IgM antibodies

LW Ling Wang
ZY Zhi Q. Yao

Last updated date: Jun 17, 2022 Views: 540 Forks: 0

original An abbreviated version of this protocol was published in Scientific Reports in Mar, 2021
Blockade of SARS-CoV-2 spike protein-mediated cell–cell fusion using COVID-19 convalescent plasma
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Hello Wei,

As we described in the Methods of the paper, The SARS-CoV-2 Spike S1-RBD IgG and IgM were qualitatively determined with a SARS-CoV-2 Spike S1-RBD IgG&IgM ELISA Detection Kit from GenScript. The following is the link of the product.

SARS-CoV-2 Spike S1-RBD IgG&IgM ELISA Detection Kit - GenScript

The manual for the kit is not available on GenScript website. 

According to the instructions of the kit, the plasma samples were centrifuged for 10min at 1000g, 1:100 diluted with sample dilution buffer provided by the kit in our paper.

 

The concentration of S1 IgG in the COVID-19 convalescent plasma was quantitatively measured using a high-sensitive SARS-CoV-2 S1 IgG ELISA kit (BioVendor, Asheville, NC). The manual version I used was 20200520 that is not available any more on the BioVendor website. The followings are the links for the product and manual for the version 20210219, which uses IU/ml instead of ng/ml to represent antibody concentration.

High-sensitivity SARS-CoV-2 S1 IgG Quantitative ELISA | BioVendor R&D

INTRODUCTION (biovendor.com)

 

I hope the above answers your questions.

Ling

 

 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Wang, L and Yao, Z(2022). Detection of SARS-CoV-2 specific IgG and IgM antibodies. Bio-protocol Preprint. bio-protocol.org/prep1726.
  2. Wang, L., Zhao, J., Nguyen, L. N. T., Adkins, J. L., Schank, M., Khanal, S., Nguyen, L. N., Dang, X., Cao, D., Thakuri, B. K. C., Lu, Z., Zhang, J., Zhang, Y., Wu, X. Y., Gazzar, M. E., Ning, S., Moorman, J. P. and Yao, Z. Q.(2021). Blockade of SARS-CoV-2 spike protein-mediated cell–cell fusion using COVID-19 convalescent plasma. Scientific Reports 0(0). DOI: 10.1038/s41598-021-84840-3

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