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Preprint

Construction of the FICA system

MW Meiyan Wang
HY Haifeng Ye

Last updated date: May 3, 2022 Views: 612 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Dec, 2021
A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation
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There are three plasmids of FICA system，including  FRL-responsive sensor vector pXY137, the light-inducible Cas12a expression vector pDL192,  and  crRNA targeted endogenous gene locus DNMT1 pZQ28. The detailed sequence of FICA is attached below.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Wang, M and Ye, H(2022). Construction of the FICA system. Bio-protocol Preprint. bio-protocol.org/prep1650.
  2. Wang, X., Dong, K., Kong, D., Zhou, Y., Yin, J., Cai, F., Wang, M. and Ye, H.(2021). A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation. Science Advances 7(50). DOI: 10.1126/sciadv.abh2358

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