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Immunohistochemistry analysis
Last updated date: Feb 23, 2022 Views: 642 Forks: 0
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Dear, Sasha Verrijt
Thank you for inquiring about the experimental method.
1. We used 5 mm thick sections for immunohistochemical analysis.
The tissue section was transferred on Microscope Slides, Diamond White Glass, 25 x 75mm, Charged, 90° Ground Edges, Yellow Frosted (Globe SCIENTIFIC INC., Cat. No. 1358Y) for IHC staining.
(This slide adheres better than general slides and does not fall off.)
2. We used TBST with 0.1% Tween 20 for all washing steps.
Kind regards,
Kwon.
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Kwon, H and Lee, F(2022). Immunohistochemistry analysis. Bio-protocol Preprint. bio-protocol.org/prep1544.
- Kwon, H., Lee, I., Yu, K. E., Cahill, S. V., Alder, K. D., Lee, S., Dussik, C. M., Back, J., Choi, J., Song, L., Kyriakides, T. R. and Lee, F. Y.(2021). Dual therapeutic targeting of intra-articular inflammation and intracellular bacteria enhances chondroprotection in septic arthritis . Science Advances 7(26). DOI: 10.1126/sciadv.abf2665
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