Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Experimental tumor dormancy

MM Masoud H. Manjili

Last updated date: Feb 23, 2022 Views: 593 Forks: 0

original An abbreviated version of this protocol was published in Oncotarget in Apr, 2018
Autophagy-deficient breast cancer shows early tumor recurrence and escape from dormancy
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite
  1. Stock solution 1 mM ADR – use dark Eppendorf tubes to make 100 uL aliquots, store at -20
  2. Seed cells at 3x10^6 per medium flask in the morning, let cells adhere (at least 4 hours), and treat with ADR high dose (1uM so add 1uL of stock solution per mL of medium – typically do 7 uL in 7 mL medium) – turn lights in hood and hood room off when adding ADR
  3. Treat x 2 hours (can do 2.5 hrs) 
  4. After 2 hours, remove medium, wash with medium then add fresh medium (7-10 mL)
  5. Check daily and change medium when needed 
Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Manjili, M(2022). Experimental tumor dormancy. Bio-protocol Preprint. bio-protocol.org/prep1547.
  2. Aqbi, H. F., Tyutyunyk-Massey, L., Keim, R. C., Butler, S. E., Thekkudan, T., Joshi, S., Smith, T. M., Bandyopadhyay, D., Idowu, M. O., Bear, H. D., Payne, K. K., Gewirtz, D. A. and Manjili, M. H.(2018). Autophagy-deficient breast cancer shows early tumor recurrence and escape from dormancy. Oncotarget 9(31). DOI: 10.18632/oncotarget.25197

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A
This protocol preprint was submitted via the "Request a Protocol" track.