Protocols shared by authors via RaP service
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Isolation and methylation profiling of cfDNA
Authors: Huiyan Luo and Rui-hua Xu
Date: Mar 12, 2020，Views：1070
Original protocol used in research article：
Circulating tumor DNA methylation profiles enable early diagnosis prognosis prediction and screening for colorectal cancer
Science Translational Medicine, Dec 31, 2019; DOI: 10.1126/scitranslmed.aax7533
Protocol for preparing demembranated sperm nuclei
Author: Xianrui Cheng
Date: Mar 11, 2020，Views：931
Original protocol used in research article：
Spontaneous emergence of cell-like organization in egg extracts
Science, Nov 1, 2019; DOI: 10.1126/science.aav7793
Protocol for making cycling egg extracts from Xenopus laevis
Author: Xianrui Cheng
Date: Mar 11, 2020，Views：1029
Original protocol used in research article：
Spontaneous emergence of cell-like organization in egg extracts
Science, Nov 1, 2019; DOI: 10.1126/science.aav7793
3,3-Bis(methylsulfonyl)propanamide experimental
Author: Clifton E. Barry
Date: Mar 11, 2020，Views：1006
Original protocol used in research article：
PE/PPE proteins mediate nutrient transport across the outer membrane of Mycobacterium tuberculosis
Science, Mar 5, 2020; DOI: 10.1126/science.aav5912
Lentiviral transduction
Authors: Saravanan Gunaseelan, Jie Yin Chua and John Jia En Chua
Date: Mar 9, 2020，Views：2788
Original protocol used in research article：
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity
eLife, Aug 27, 2019; DOI: 10.7554/eLife.47498
Image analyses
Author: Sari Tojkander
Date: Mar 9, 2020，Views：1070
Original protocol used in research article：
Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly
eLife, Dec 10, 2015; DOI: 10.7554/eLife.06126
Collagen gel chamber assembly
Author: Enas Abu-Shah
Date: Mar 6, 2020，Views：1030
Original protocol used in research article：
A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells
eLife, Sep 25, 2019; DOI: 10.7554/eLife.48221
Tissue contrasting for µ-CT scanning
Author: Marketa Kaucka
Date: Mar 6, 2020，Views：1061
Original protocol used in research article：
Signals from the brain and olfactory epithelium control shaping of the mammalian nasal capsule cartilage
eLife, Jun 13, 2018; DOI: 10.7554/eLife.34465
SNARE constructs
Authors: Junyi Jiao, Frederick M Hughson and Yongli Zhang
Date: Mar 5, 2020，Views：1076
Original protocol used in research article：
Munc18-1 catalyzes neuronal SNARE assembly by templating SNARE association
eLife, Dec 12, 2018; DOI: 10.7554/eLife.41771
Tagmentation procedure for ATAC-seq of sorted GFP+ endothelial cells
Authors: Mark F Sabbagh and Jeremy Nathans
Date: Mar 4, 2020，Views：1517
Original protocol used in research article：
A genome-wide view of the de-differentiation of central nervous system endothelial cells in culture
eLife, Jan 8, 2020; DOI: 10.7554/eLife.51276
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