Protocols shared by authors via RaP service
Journal
Development of the aspiration-assisted bioprinter
Author: Ibrahim T. Ozbolat
Date: Mar 25, 2020，Views：955
Original protocol used in research article：
Aspiration-assisted bioprinting for precise positioning of biologics
Science Advances, Mar 5, 2020; DOI: 10.1126/sciadv.aaw5111
Cell culture
Authors: Vladimir I Gelfand and Rosalind Norkett
Date: Mar 20, 2020，Views：990
Original protocol used in research article：
Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding
eLife, Feb 5, 2020; DOI: 10.7554/eLife.52009
Sample preparation for fPALM imaging
Authors: Nathan A McDonald and Kathleen L Gould
Date: Mar 17, 2020，Views：994
Original protocol used in research article：
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring
eLife, Sep 15, 2017; DOI: 10.7554/eLife.28865
Measurement of acetyl-CoA
Author: Pamela Maher
Date: Mar 17, 2020，Views：1380
Original protocol used in research article：
Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866
Isolation and cell culture of primary alveolar macrophages
Authors: Einar Kleinhans Krogsaeter, Barbara Spix, Elisabeth Butz, Anna Scotto Rosato and Christian Grimm
Date: Mar 17, 2020，Views：1710
Original protocol used in research article：
Selective agonist of TRPML2 reveals direct role in chemokine release from innate immune cells
eLife, Nov 27, 2018; DOI: 10.7554/eLife.39720
Integration method (with replacement of go omissions)
Author: Frederick Verbruggen
Date: Mar 17, 2020，Views：1611
Original protocol used in research article：
A consensus guide to capturing the ability to inhibit actions and impulsive behaviors in the stop-signal task
eLife, Apr 29, 2019; DOI: 10.7554/eLife.46323
Immunofluorescence
Author: Nicolas Damond
Date: Mar 17, 2020，Views：1042
Original protocol used in research article：
Blockade of glucagon signaling prevents or reverses diabetes onset only if residual β-cells persist
eLife, Apr 19, 2016; DOI: 10.7554/eLife.13828
Estimation of the phase and amplitude envelope
Authors: Jessica K Nadalin and Mark A Kramer
Date: Mar 16, 2020，Views：1306
Original protocol used in research article：
A statistical framework to assess cross-frequency coupling while accounting for confounding analysis effects
eLife, Oct 16, 2019; DOI: 10.7554/eLife.44287
NPM1-NeonGreen
Author: C. Zechner
Date: Mar 13, 2020，Views：874
Original protocol used in research article：
Phase separation provides a mechanism to reduce noise in cells
Science, Jan 23, 2020; DOI: 10.1126/science.aav6691
Western blot
Authors: Valerio Azzimato and Myriam Aouadi
Date: Mar 13, 2020，Views：1249
Original protocol used in research article：
Liver macrophages inhibit the endogenous antioxidant response in obesity-associated insulin resistance
Science Translational Medicine, Feb 25, 2020; DOI: 10.1126/scitranslmed.aaw9709
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