Protocols shared by authors via RaP service
Journal
α-syn purification
Author: Smriti Sangwan
Date: Feb 20, 2020，Views：1193
Original protocol used in research article：
Inhibition of synucleinopathic seeding by rationally designed inhibitors
eLife, Jan 2, 2020; DOI: 10.7554/eLife.46775
mRNA preparation
Author: Maaike Welling
Date: Feb 20, 2020，Views：3612
Original protocol used in research article：
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
eLife, Jan 21, 2019; DOI: 10.7554/eLife.44491
In vitro kinase assay using immunoprecipitated Cdk5 from SCN
Authors: Andrea Brenna and Urs Albrecht
Date: Feb 20, 2020，Views：1266
Original protocol used in research article：
Cyclin-dependent kinase 5 (CDK5) regulates the circadian clock
eLife, Nov 5, 2019; DOI: 10.7554/eLife.50925
Live imaging of tracheal invasion into pupal IFMs
Authors: Stefan Luschnig and Julia Sauerwald
Date: Feb 19, 2020，Views：1230
Original protocol used in research article：
Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857
Immunofluorescence and imaging
Author: Aimin Peng
Date: Feb 18, 2020，Views：1126
Original protocol used in research article：
Kinesin Kif2C in regulation of DNA double strand break dynamics and repair
eLife, Jan 17, 2020; DOI: 10.7554/eLife.53402
Tissue processing for small capillary immunochemistry
Authors: Guillermo Luxán, Mara E Pitulescu and Ralf H Adams
Date: Feb 18, 2020，Views：1236
Original protocol used in research article：
Endothelial EphB4 maintains vascular integrity and transport function in adult heart
eLife, Nov 29, 2019; DOI: 10.7554/eLife.45863
Trypsin digestion
Author: Giulia Bertolin
Date: Feb 17, 2020，Views：1689
Original protocol used in research article：
Aurora kinase A localises to mitochondria to control organelle dynamics and energy production
eLife, Aug 2, 2018; DOI: 10.7554/eLife.38111
Cell culture
Author: Gabriela Dankova
Date: Feb 13, 2020，Views：1290
Original protocol used in research article：
Novel genetic loci affecting facial shape variation in humans
eLife, Nov 26, 2019; DOI: 10.7554/eLife.49898
Radiolabelled nutrient uptake for suspension cells
Author: Linda V Sinclair
Date: Feb 12, 2020，Views：1216
Original protocol used in research article：
Antigen receptor control of methionine metabolism in T cells
eLife, Mar 27, 2019; DOI: 10.7554/eLife.44210
Single-cell transcriptomics platform: 10x Chromium
Authors: Ralph Patrick, Robert E Nordon and Richard P Harvey
Date: Feb 11, 2020，Views：1758
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
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