Protocols shared by authors via RaP service
Journal
Fluorometry of solubilized cell extracts
Author: Seksiri Arttamangkul
Date: Dec 16, 2019，Views：998
Original protocol used in research article：
Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry
eLife, Oct 7, 2019; DOI: 10.7554/eLife.49319
Live-cell imaging
Author: Elçin Ünal
Date: Dec 16, 2019，Views：1097
Original protocol used in research article：
Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast
eLife, Aug 9, 2019; DOI: 10.7554/eLife.47156
Quantification of neural crest contributions to the ventricular myocardium and regeneration
Author: Marianne E Bronner
Date: Dec 14, 2019，Views：1291
Original protocol used in research article：
Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
eLife, Aug 8, 2019; DOI: 10.7554/eLife.47929
Flow cytometry blood staining protocol for BLT humanized mice
Author: Collin Kieffer
Date: Dec 13, 2019，Views：1189
Original protocol used in research article：
Mechanisms of virus dissemination in bone marrow of HIV-1–infected humanized BLT mice
eLife, Oct 28, 2019; DOI: 10.7554/eLife.46916
Droplet capture of single nuclei, cDNA sequencing and data analysis
Author: Nicholas Ryba
Date: Dec 13, 2019，Views：1560
Original protocol used in research article：
Stereotyped transcriptomic transformation of somatosensory neurons in response to injury
eLife, Oct 8, 2019; DOI: 10.7554/eLife.49679
Generation of transgenic fish and chemical treatment
Author: Bo Zhang
Date: Dec 13, 2019，Views：1068
Original protocol used in research article：
One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish
eLife, Oct 30, 2019; DOI: 10.7554/eLife.48081
Metabolite extraction and LC-MS analysis
Author: Eugene I Shakhnovich
Date: Dec 12, 2019，Views：1084
Original protocol used in research article：
Adaptation to mutational inactivation of an essential gene converges to an accessible suboptimal fitness peak
eLife, Oct 1, 2019; DOI: 10.7554/eLife.50509
ChIP-Sequencing
Author: Neal A Deluca
Date: Dec 12, 2019，Views：1481
Original protocol used in research article：
Herpes simplex viral nucleoprotein creates a competitive transcriptional environment facilitating robust viral transcription and host shut off
eLife, Oct 22, 2019; DOI: 10.7554/eLife.51109
Four-quadrant behavioral assay
Authors: Christopher J Potter and Chun-Chieh Lin
Date: Dec 12, 2019，Views：1168
Original protocol used in research article：
Food odors trigger Drosophila males to deposit a pheromone that guides aggregation and female oviposition decisions
eLife, Sep 30, 2015; DOI: 10.7554/eLife.08688
In silico analyses of SecA pore dynamics
Author: Ian Collinson
Date: Dec 12, 2019，Views：1097
Original protocol used in research article：
HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon
eLife, Jul 10, 2019; DOI: 10.7554/eLife.47402
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