Protocols shared by authors via RaP service
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Single molecule RNA fluorescent in situ hybridization (FISH) and imaging
Author: Michele L Lemons
Date: Jan 6, 2020，Views：1079
Original protocol used in research article：
Neurexin directs partner-specific synaptic connectivity in C. elegans
eLife, Jul 24, 2018; DOI: 10.7554/eLife.35692
Stability calculations
Author: Amelie Stein
Date: Dec 30, 2019，Views：1147
Original protocol used in research article：
Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome
eLife, Nov 7, 2019; DOI: 10.7554/eLife.49138
FRET measurement
Authors: Wanli Liu, Wei Chen and Jizhong Lou
Date: Dec 29, 2019，Views：1255
Original protocol used in research article：
FcγRIIB-I232T polymorphic change allosterically suppresses ligand binding
eLife, Jul 25, 2019; DOI: 10.7554/eLife.46689
Laser speckle perfusion measurements of brain
Author: Stefan Offermanns
Date: Dec 29, 2019，Views：2289
Original protocol used in research article：
Myogenic vasoconstriction requires G12/G13 and LARG to maintain local and systemic vascular resistance
eLife, Sep 24, 2019; DOI: 10.7554/eLife.49374
5’/3’ RACE
Author: Judith Wexler
Date: Dec 24, 2019，Views：1307
Original protocol used in research article：
Hemimetabolous insects elucidate the origin of sexual development via alternative splicing
eLife, Sep 3, 2019; DOI: 10.7554/eLife.47490
β-galactosidase detection in embryos and tissues using X-Gal
Author: Shahragim Tajbakhsh
Date: Dec 22, 2019，Views：993
Original protocol used in research article：
A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle
eLife, Sep 19, 2019; DOI: 10.7554/eLife.47460
Electrophoretic mobility shift assay
Author: Yan Zhang
Date: Dec 19, 2019，Views：1102
Original protocol used in research article：
Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
eLife, Aug 6, 2019; DOI: 10.7554/eLife.48725
Single cell isolation and flow cytometry for scRNA-Seq from utricular sensory epithelia
Authors: Mirko Scheibinger, Stefan Heller and Peter G Barr-Gillespie
Date: Dec 17, 2019，Views：1155
Original protocol used in research article：
Single-cell proteomics reveals changes in expression during hair-cell development
eLife, Nov 4, 2019; DOI: 10.7554/eLife.50777
Intact protein mass spectrometry
Author: Michael A Marletta
Date: Dec 17, 2019，Views：1743
Original protocol used in research article：
Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy
eLife, Sep 30, 2019; DOI: 10.7554/eLife.50634
Natural transformation assay
Author: Melanie Blokesch
Date: Dec 17, 2019，Views：1230
Original protocol used in research article：
Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae
eLife, Sep 3, 2019; DOI: 10.7554/eLife.48212
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