Protocols shared by authors via RaP service
Journal
Cell culture
Author: Gabriela Dankova
Date: Feb 13, 2020，Views：1269
Original protocol used in research article：
Novel genetic loci affecting facial shape variation in humans
eLife, Nov 26, 2019; DOI: 10.7554/eLife.49898
Radiolabelled nutrient uptake for suspension cells
Author: Linda V Sinclair
Date: Feb 12, 2020，Views：1166
Original protocol used in research article：
Antigen receptor control of methionine metabolism in T cells
eLife, Mar 27, 2019; DOI: 10.7554/eLife.44210
Single-cell transcriptomics platform: 10x Chromium
Authors: Ralph Patrick, Robert E Nordon and Richard P Harvey
Date: Feb 11, 2020，Views：1735
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
Mouse tracking
Authors: Yair Shemesh, Oren Forkosh, Alon Chen and Elad Schneidman
Date: Feb 2, 2020，Views：1686
Original protocol used in research article：
High-order social interactions in groups of mice
eLife, Sep 3, 2013; DOI: 10.7554/eLife.00759
Calculation of tunnel volume
Authors: Fatima Alejandra Pardo Avila, Renuka Kudva and Gunnar von Heijne
Date: Jan 30, 2020，Views：1799
Original protocol used in research article：
The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
eLife, Nov 26, 2018; DOI: 10.7554/eLife.36326
Detailed BrdU immuno protocol with anti-YFP pre-staining
Authors: Zoe Christenson Wick, Esther Krook-Magnuson and Madison Tetzlaff
Date: Jan 27, 2020，Views：1055
Original protocol used in research article：
Novel long-range inhibitory nNOS-expressing hippocampal cells
eLife, Oct 14, 2019; DOI: 10.7554/eLife.46816
Pull-down and identification of the ligand
Author: Timo Vögtle
Date: Jan 23, 2020，Views：1276
Original protocol used in research article：
Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B
eLife, Aug 22, 2019; DOI: 10.7554/eLife.46840
MWC model simulations
Authors: Olga Rapp and Ofer Yifrach
Date: Jan 20, 2020，Views：1191
Original protocol used in research article：
Evolutionary and functional insights into the mechanism underlying body-size-related adaptation of mammalian hemoglobin
eLife, Oct 24, 2019; DOI: 10.7554/eLife.47640
Immunohistochemical analysis of early passage cultures
Author: Krishna Kalyan Kolluri
Date: Jan 14, 2020，Views：1300
Original protocol used in research article：
Loss of functional BAP1 augments sensitivity to TRAIL in cancer cells
eLife, Jan 18, 2018; DOI: 10.7554/eLife.30224
Chromatin and whole cell Flow cytometry
Authors: Jacob Peter Matson and Jeanette Gowen Cook
Date: Jan 14, 2020，Views：1282
Original protocol used in research article：
Rapid DNA replication origin licensing protects stem cell pluripotency
eLife, Nov 17, 2017; DOI: 10.7554/eLife.30473
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