The protocol in M&M contains the info on hardware, buffer and materials. It also contains the exact concentrations of solutions used, flow rates and time of incubation as well as the sequence of events. I would need your help on what is missing in the protocol and am happy to provide that information if I could get feedback on exactly what you are looking for.
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How to cite:
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
Pitsawong, W., Buosi, V., Otten, R., Agafonov, R. V., Zorba, A., Kern, N., Kutter, S., Kern, G., Pádua, R. A., Meniche, X. and Kern, D.(2018). Dynamics of human protein kinase Aurora A linked to drug selectivity. eLife. DOI: 10.7554/eLife.36656
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