Protocols shared by authors via RaP service
Journal
Immunofluorescence
Author: Nicolas Damond
Date: Mar 17, 2020，Views：1077
Original protocol used in research article：
Blockade of glucagon signaling prevents or reverses diabetes onset only if residual β-cells persist
eLife, Apr 19, 2016; DOI: 10.7554/eLife.13828
Estimation of the phase and amplitude envelope
Authors: Jessica K Nadalin and Mark A Kramer
Date: Mar 16, 2020，Views：1359
Original protocol used in research article：
A statistical framework to assess cross-frequency coupling while accounting for confounding analysis effects
eLife, Oct 16, 2019; DOI: 10.7554/eLife.44287
NPM1-NeonGreen
Author: C. Zechner
Date: Mar 13, 2020，Views：900
Original protocol used in research article：
Phase separation provides a mechanism to reduce noise in cells
Science, Jan 23, 2020; DOI: 10.1126/science.aav6691
Western blot
Authors: Valerio Azzimato and Myriam Aouadi
Date: Mar 13, 2020，Views：1297
Original protocol used in research article：
Liver macrophages inhibit the endogenous antioxidant response in obesity-associated insulin resistance
Science Translational Medicine, Feb 25, 2020; DOI: 10.1126/scitranslmed.aaw9709
Isolation and methylation profiling of cfDNA
Authors: Huiyan Luo and Rui-hua Xu
Date: Mar 12, 2020，Views：1110
Original protocol used in research article：
Circulating tumor DNA methylation profiles enable early diagnosis prognosis prediction and screening for colorectal cancer
Science Translational Medicine, Dec 31, 2019; DOI: 10.1126/scitranslmed.aax7533
Protocol for preparing demembranated sperm nuclei
Author: Xianrui Cheng
Date: Mar 11, 2020，Views：969
Original protocol used in research article：
Spontaneous emergence of cell-like organization in egg extracts
Science, Nov 1, 2019; DOI: 10.1126/science.aav7793
Protocol for making cycling egg extracts from Xenopus laevis
Author: Xianrui Cheng
Date: Mar 11, 2020，Views：1098
Original protocol used in research article：
Spontaneous emergence of cell-like organization in egg extracts
Science, Nov 1, 2019; DOI: 10.1126/science.aav7793
3,3-Bis(methylsulfonyl)propanamide experimental
Author: Clifton E. Barry
Date: Mar 11, 2020，Views：1053
Original protocol used in research article：
PE/PPE proteins mediate nutrient transport across the outer membrane of Mycobacterium tuberculosis
Science, Mar 5, 2020; DOI: 10.1126/science.aav5912
Lentiviral transduction
Authors: Saravanan Gunaseelan, Jie Yin Chua and John Jia En Chua
Date: Mar 9, 2020，Views：2845
Original protocol used in research article：
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity
eLife, Aug 27, 2019; DOI: 10.7554/eLife.47498
Image analyses
Author: Sari Tojkander
Date: Mar 9, 2020，Views：1114
Original protocol used in research article：
Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly
eLife, Dec 10, 2015; DOI: 10.7554/eLife.06126
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