Protocols shared by authors via RaP service
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Image analyses
Author: Sari Tojkander
Date: Mar 9, 2020，Views：1113
Original protocol used in research article：
Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly
eLife, Dec 10, 2015; DOI: 10.7554/eLife.06126
Collagen gel chamber assembly
Author: Enas Abu-Shah
Date: Mar 6, 2020，Views：1084
Original protocol used in research article：
A tissue-like platform for studying engineered quiescent human T-cells’ interactions with dendritic cells
eLife, Sep 25, 2019; DOI: 10.7554/eLife.48221
Tissue contrasting for µ-CT scanning
Author: Marketa Kaucka
Date: Mar 6, 2020，Views：1111
Original protocol used in research article：
Signals from the brain and olfactory epithelium control shaping of the mammalian nasal capsule cartilage
eLife, Jun 13, 2018; DOI: 10.7554/eLife.34465
SNARE constructs
Authors: Junyi Jiao, Frederick M Hughson and Yongli Zhang
Date: Mar 5, 2020，Views：1130
Original protocol used in research article：
Munc18-1 catalyzes neuronal SNARE assembly by templating SNARE association
eLife, Dec 12, 2018; DOI: 10.7554/eLife.41771
Tagmentation procedure for ATAC-seq of sorted GFP+ endothelial cells
Authors: Mark F Sabbagh and Jeremy Nathans
Date: Mar 4, 2020，Views：1585
Original protocol used in research article：
A genome-wide view of the de-differentiation of central nervous system endothelial cells in culture
eLife, Jan 8, 2020; DOI: 10.7554/eLife.51276
Assessment of mortality
Author: Xia Li
Date: Mar 4, 2020，Views：1311
Original protocol used in research article：
Longitudinal trajectories, correlations and mortality associations of nine biological ages across 20-years follow-up
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51507
Fly strains
Authors: Fengwei Yu and menglong Rui
Date: Mar 3, 2020，Views：1148
Original protocol used in research article：
Patronin governs minus-end-out orientation of dendritic microtubules to promote dendrite pruning in Drosophila
eLife, Mar 28, 2019; DOI: 10.7554/eLife.39964
Genome editing by CRISPR-Cas9
Authors: Rui Jia and Juan S Bonifacino
Date: Mar 2, 2020，Views：1246
Original protocol used in research article：
Negative regulation of autophagy by UBA6-BIRC6–mediated ubiquitination of LC3
eLife, Nov 6, 2019; DOI: 10.7554/eLife.50034
Construction of the RapiDeg yeast strain
Author: Lu Zhu
Date: Feb 26, 2020，Views：1179
Original protocol used in research article：
ESCRTs function directly on the lysosome membrane to downregulate ubiquitinated lysosomal membrane proteins
eLife, Jun 29, 2017; DOI: 10.7554/eLife.26403
Bayesian inference of progenitor types
Authors: Alfredo Llorca and Oscar Marin
Date: Feb 26, 2020，Views：1138
Original protocol used in research article：
A stochastic framework of neurogenesis underlies the assembly of neocortical cytoarchitecture
eLife, Nov 18, 2019; DOI: 10.7554/eLife.51381
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