Protocols shared by authors via RaP service
Journal
Juxtacellular labelling of single neurons
Author: Navid Farassat
Date: Feb 21, 2020，Views：1192
Original protocol used in research article：
In vivo functional diversity of midbrain dopamine neurons within identified axonal projections
eLife, Oct 3, 2019; DOI: 10.7554/eLife.48408
Chromatin immunoprecipitation (ChIP)
Authors: Xuehua Zhong and Xiangsong Chen
Date: Feb 20, 2020，Views：1329
Original protocol used in research article：
POWERDRESS interacts with HISTONE DEACETYLASE 9 to promote aging in Arabidopsis
eLife, Nov 22, 2016; DOI: 10.7554/eLife.17214
Automatic determination of optimal cluster number
Author: Jakob Nikolas Kather
Date: Feb 20, 2020，Views：1232
Original protocol used in research article：
Topography of cancer-associated immune cells in human solid tumors
eLife, Sep 4, 2018; DOI: 10.7554/eLife.36967
α-syn purification
Author: Smriti Sangwan
Date: Feb 20, 2020，Views：1172
Original protocol used in research article：
Inhibition of synucleinopathic seeding by rationally designed inhibitors
eLife, Jan 2, 2020; DOI: 10.7554/eLife.46775
mRNA preparation
Author: Maaike Welling
Date: Feb 20, 2020，Views：3587
Original protocol used in research article：
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
eLife, Jan 21, 2019; DOI: 10.7554/eLife.44491
In vitro kinase assay using immunoprecipitated Cdk5 from SCN
Authors: Andrea Brenna and Urs Albrecht
Date: Feb 20, 2020，Views：1242
Original protocol used in research article：
Cyclin-dependent kinase 5 (CDK5) regulates the circadian clock
eLife, Nov 5, 2019; DOI: 10.7554/eLife.50925
Live imaging of tracheal invasion into pupal IFMs
Authors: Stefan Luschnig and Julia Sauerwald
Date: Feb 19, 2020，Views：1206
Original protocol used in research article：
Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857
Immunofluorescence and imaging
Author: Aimin Peng
Date: Feb 18, 2020，Views：1108
Original protocol used in research article：
Kinesin Kif2C in regulation of DNA double strand break dynamics and repair
eLife, Jan 17, 2020; DOI: 10.7554/eLife.53402
Tissue processing for small capillary immunochemistry
Authors: Guillermo Luxán, Mara E Pitulescu and Ralf H Adams
Date: Feb 18, 2020，Views：1224
Original protocol used in research article：
Endothelial EphB4 maintains vascular integrity and transport function in adult heart
eLife, Nov 29, 2019; DOI: 10.7554/eLife.45863
Trypsin digestion
Author: Giulia Bertolin
Date: Feb 17, 2020，Views：1655
Original protocol used in research article：
Aurora kinase A localises to mitochondria to control organelle dynamics and energy production
eLife, Aug 2, 2018; DOI: 10.7554/eLife.38111
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