Protocols shared by authors via RaP service
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Immunofluorescence staining - Vibratome Sections for Oligodendrocyte morphological analysis
Author: Matthew Swire
Date: Dec 6, 2019，Views：10049
Original protocol used in research article：
Endothelin signalling mediates experience-dependent myelination in the CNS
eLife, Oct 28, 2019; DOI: 10.7554/eLife.49493
General Protocol for Resistance Assays in Liquid Media
Author: Nathaniel J Traaseth
Date: Dec 4, 2019，Views：1099
Original protocol used in research article：
Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations
eLife, Oct 22, 2019; DOI: 10.7554/eLife.48909
C2C12 culture and generation of Gene-modified C2C12 cells
Author: Lin Mei
Date: Dec 4, 2019，Views：1160
Original protocol used in research article：
A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome
eLife, Sep 24, 2019; DOI: 10.7554/eLife.49180
Quantification of tracheal density in IFMs
Author: Stefan Luschnig
Date: Dec 4, 2019，Views：1158
Original protocol used in research article：
Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857
Growth chamber experiments
Author: Steven Penfield
Date: Dec 4, 2019，Views：1155
Original protocol used in research article：
Flowering time and seed dormancy control use external coincidence to generate life history strategy
eLife, Mar 31, 2015; DOI: 10.7554/eLife.05557
Isolation of skeletal muscle-derived mononuclear cells
Author: So-ichiro Fukada
Date: Dec 3, 2019，Views：1051
Original protocol used in research article：
Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle
eLife, Sep 23, 2019; DOI: 10.7554/eLife.48284
Purification of mammalian dynein-dynactin
Author: Ahmet Yildiz
Date: Dec 3, 2019，Views：1127
Original protocol used in research article：
Kinesin and dynein use distinct mechanisms to bypass obstacles
eLife, Sep 9, 2019; DOI: 10.7554/eLife.48629
In vitro differentiation of mESCs in embryoid bodies
Author: Lars Schomacher
Date: Dec 3, 2019，Views：1190
Original protocol used in research article：
NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress
eLife, Sep 30, 2019; DOI: 10.7554/eLife.49044
Platelet isolation and quantification
Author: Mercedes Costell
Date: Dec 1, 2019，Views：1091
Original protocol used in research article：
The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes
eLife, Jan 16, 2017; DOI: 10.7554/eLife.22264
Preparation of mini-Gs heterotrimer
Author: Christopher Gordon Tate
Date: Dec 1, 2019，Views：1691
Original protocol used in research article：
Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein
eLife, May 4, 2018; DOI: 10.7554/eLife.35946
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