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A high-quality database for basic life science protocols
Protocols
Research fields
Biochemistry (1256)
Biophysics (122)
Cancer Biology (401)
Cell Biology (1890)
Developmental Biology (289)
Immunology (564)
Microbiology (1061)
Molecular Biology (1014)
Neuroscience (612)
Plant Science (982)
Stem Cell (219)
Systems Biology (281)
Bio-protocol journal
Special issues
Lab protocol hub
Request a Protocol
Submit
Submit a protocol
Author guidelines
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Virulence
ESC-derived motor neurons
Author:
Esteban O. Mazzoni
Date: Nov 21, 2019,Views:1218
Original protocol used in research article:
Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons
eLife, Jun 3, 2019; DOI:
10.7554/eLife.44423
Influenza A genome sequencing
Author:
Nacho Mena
Date: Nov 20, 2019,Views:1606
Original protocol used in research article:
Origins of the 2009 H1N1 influenza pandemic in swine in Mexico
eLife, Jun 28, 2016; DOI:
10.7554/eLife.16777
Desiccation survival
Author:
Jennifer Sun
Date: Nov 18, 2019,Views:1067
Original protocol used in research article:
Humidity response depends on the small soluble protein Obp59a in
Drosophila
eLife, Sep 19, 2018; DOI:
10.7554/eLife.39249
Observation and motility assay of zebrafish spermatozoa
Authors:
Masahide Kikkawa
and Hiroshi Yamaguchi
Date: Nov 18, 2019,Views:1238
Original protocol used in research article:
Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly
eLife, May 9, 2018; DOI:
10.7554/eLife.36979
Western blots
Author:
Joel Hrit
Date: Nov 18, 2019,Views:1100
Original protocol used in research article:
OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function in development
eLife, Oct 16, 2018; DOI:
10.7554/eLife.34870
4TU metabolic labeling and RNA analysis
Author:
Leon Chan
Date: Nov 18, 2019,Views:1461
Original protocol used in research article:
Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability
eLife, Sep 7, 2018; DOI:
10.7554/eLife.32536
Immunofluorescence assay for 5hmc
Author:
Vladimir Camarena
Date: Nov 18, 2019,Views:1371
Original protocol used in research article:
cAMP signaling regulates DNA hydroxymethylation by augmenting the intracellular labile ferrous iron pool
eLife, Dec 14, 2017; DOI:
10.7554/eLife.29750
GFP dropout screening
Author:
Jason Sheltzer
Date: Nov 18, 2019,Views:1413
Original protocol used in research article:
CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going clinical trials
eLife, Mar 24, 2017; DOI:
10.7554/eLife.24179
Rotarod (Ugo Basile Biological Research Apparatus)
Author:
Cristian A Lasagna-Reeves
Date: Nov 18, 2019,Views:1974
Original protocol used in research article:
Ataxin-1 oligomers induce local spread of pathology and decreasing them by passive immunization slows Spinocerebellar ataxia type 1 phenotypes
eLife, Dec 17, 2015; DOI:
10.7554/eLife.10891
Detailed protocol for mouse adipose tissue protein extraction
Authors:
Yu An
and Philipp E Scherer
Date: Nov 18, 2019,Views:4369
Original protocol used in research article:
Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis
eLife, Mar 29, 2017; DOI:
10.7554/eLife.24071
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