Protocols shared by authors via RaP service
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Aversive phototaxic suppression (APS)
Authors: Chunghun Lim and Yoonhee Ki
Date: Apr 21, 2020，Views：1732
Original protocol used in research article：
Sleep-promoting effects of threonine link amino acid metabolism in Drosophila neuron to GABAergic control of sleep drive
eLife, Jul 17, 2019; DOI: 10.7554/eLife.40593
Generation and LED light photoactivation of Opto-receptors
Authors: Anassuya Ramachandran and Caroline S Hill
Date: Apr 20, 2020，Views：1156
Original protocol used in research article：
TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition
eLife, Jan 29, 2018; DOI: 10.7554/eLife.31756
Magnet-assisted flow cytometry of in vivo tumors to quantitate cell-specific responses to magnetic iron oxide nanoparticles
Authors: Preethi Korangath and Robert Ivkov
Date: Apr 17, 2020，Views：1154
Original protocol used in research article：
Nanoparticle interactions with immune cells dominate tumor retention and induce T cell–mediated tumor suppression in models of breast cancer
Science Advances, Mar 24, 2020; DOI: 10.1126/sciadv.aay1601
RNA seq data analysis
Author: Ilaria Granata
Date: Apr 15, 2020，Views：1143
Original protocol used in research article：
Human axial progenitors generate trunk neural crest cells in vitro
eLife, Aug 10, 2018; DOI: 10.7554/eLife.35786
Immunofluorescent staining of mouse embryonic tissues
Authors: Jingyue Xu and Rulang Jiang
Date: Apr 14, 2020，Views：1012
Original protocol used in research article：
Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
eLife, Jan 14, 2019; DOI: 10.7554/eLife.40315
Purification of APP C100-FLAG substrate
Authors: David M Bolduc, Michael S Wolfe and Dennis J Selkoe
Date: Apr 14, 2020，Views：1209
Original protocol used in research article：
The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase
eLife, Aug 31, 2016; DOI: 10.7554/eLife.17578
Step-by-step lamina propria cell isolation protocol for H. polygyrus infected intestines
Author: Johannes U Mayer
Date: Apr 13, 2020，Views：1461
Original protocol used in research article：
High-dimensional analysis of intestinal immune cells during helminth infection
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51678
β-Arrestin recruitment
Authors: Alix A. J. Rouault and Julien A. Sebag
Date: Apr 13, 2020，Views：1156
Original protocol used in research article：
The GPCR accessory protein MRAP2 regulates both biased signaling and constitutive activity of the ghrelin receptor GHSR1a
Science Signaling, Jan 6, 2020; DOI: 10.1126/scisignal.aax4569
Whole mount immunostaining
Author: Manju Sharma
Date: Apr 13, 2020，Views：1293
Original protocol used in research article：
Identification of EOMES-expressing spermatogonial stem cells and their regulation by PLZF
eLife, May 31, 2019; DOI: 10.7554/eLife.43352
Cell surface ELISA
Author: Amanda N Hayward
Date: Apr 13, 2020，Views：1469
Original protocol used in research article：
A toolkit for studying cell surface shedding of diverse transmembrane receptors
eLife, Jun 7, 2019; DOI: 10.7554/eLife.46983
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