Protocols shared by authors via RaP service
Journal
In situ hybridization protocol
Author: Peng Huang
Date: Dec 11, 2019，Views：1060
Original protocol used in research article：
Notch signalling maintains Hedgehog responsiveness via a Gli-dependent mechanism during spinal cord patterning in zebrafish
eLife, Aug 27, 2019; DOI: 10.7554/eLife.49252
3D reconstruction (and others)
Author: David M. Miller
Date: Dec 11, 2019，Views：1079
Original protocol used in research article：
C. elegans neurons have functional dendritic spines
eLife, Oct 4, 2019; DOI: 10.7554/eLife.47918
Genotyping
Author: Cunming Duan
Date: Dec 11, 2019，Views：1163
Original protocol used in research article：
Cell-autonomous regulation of epithelial cell quiescence by calcium channel Trpv6
eLife, Sep 17, 2019; DOI: 10.7554/eLife.48003
Analysis of spatial scale sizes
Author: Michael Peer
Date: Dec 11, 2019，Views：1056
Original protocol used in research article：
Processing of different spatial scales in the human brain
eLife, Sep 10, 2019; DOI: 10.7554/eLife.47492
Cuticle preparations
Authors: Yoseop Yoon and Urs Schmidt-Ott
Date: Dec 11, 2019，Views：1333
Original protocol used in research article：
Embryo polarity in moth flies and mosquitoes relies on distinct old genes with localized transcript isoforms
eLife, Oct 8, 2019; DOI: 10.7554/eLife.46711
Generation of HeLa KO cells with CRISPR
Authors: Florian Ullrich and Thomas J Jentsch
Date: Dec 11, 2019，Views：1166
Original protocol used in research article：
Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels
eLife, Jul 18, 2019; DOI: 10.7554/eLife.49187
Imaging Orc1 cellular dynamics in Drosophila S2 cells
Author: Michael R Botchan
Date: Dec 10, 2019，Views：1062
Original protocol used in research article：
A new class of disordered elements controls DNA replication through initiator self-assembly
eLife, Sep 27, 2019; DOI: 10.7554/eLife.48562
Large-scale metabolome analysis
Authors: Pamela Maher and Antonio Currais
Date: Dec 9, 2019，Views：1459
Original protocol used in research article：
Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866
Respiration measurements
Author: David C Chan
Date: Dec 8, 2019，Views：1141
Original protocol used in research article：
Mitochondrial fusion is required for spermatogonial differentiation and meiosis
eLife, Oct 9, 2019; DOI: 10.7554/eLife.51601
Drosha cleavage assay
Author: Tsui Han Loo
Date: Dec 6, 2019，Views：1169
Original protocol used in research article：
The mammalian LINC complex component SUN1 regulates muscle regeneration by modulating drosha activity
eLife, Nov 5, 2019; DOI: 10.7554/eLife.49485
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