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MG132 treatment of worms

YL Ying Liu

Last updated date: May 2, 2020 Views: 1147 Forks: 0

original An abbreviated version of this protocol was published in eLife in Jan, 2019
SUMO peptidase ULP-4 regulates mitochondrial UPR-mediated innate immunity and lifespan extension
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1. Worms are raised on NGM agar plates and fed with E.coli for 2 days, until they reach L4 stage.

2. Overnight cultured E.coli is centrifuged at 5000g for 5 min and the supernatant is discarded.

3. E.coli is resuspended by M9 buffer to OD600=1. 0.5mL M9 containing E.coli is added to a 1.5mL Eppendorf tube.

4. MG132 (sc-201270, Santa Cruz) or DMSO is added into the Eppendorf tubes. The final concentration of MG132 is100μM.

5. Worms are transferred into 1.5 mL microcentrifuge tubes and rotated on a vertical tube rotator at 20℃ for 24h.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Liu, Y(2020). MG132 treatment of worms. Bio-protocol Preprint. bio-protocol.org/prep295.
  2. Gao, K., Li, Y., Hu, S. and Liu, Y.(2019). SUMO peptidase ULP-4 regulates mitochondrial UPR-mediated innate immunity and lifespan extension. eLife. DOI: 10.7554/eLife.41792

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