Protocols shared by authors via RaP service
Journal
Genomic analysis
Authors: Xinyu Ling and Tao Liu
Date: Jun 22, 2020，Views：1014
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Isolation, culture, and lentiviral transduction of primary human T cells
Authors: Yingxiao Wang and ziliang huang
Date: Jun 22, 2020，Views：1007
Original protocol used in research article：
Engineering light-controllable CAR T cells for cancer immunotherapy
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aay9209
Loading of TH588 and Ce6 to SCLMs
Authors: Lingzhi Zhao, Juanjuan Peng and Yanli Zhao
Date: Jun 22, 2020，Views：930
Original protocol used in research article：
MTH1 inhibitor amplifies the lethality of reactive oxygen species to tumor in photodynamic therapy
Science Advances, Mar 4, 2020; DOI: 10.1126/sciadv.aaz0575
QiSant Biomarker Measurement in Urine Samples
Authors: Joshua Y.C. Yang and Minnie M. Sarwal
Date: Jun 19, 2020，Views：1048
Original protocol used in research article：
A urine score for noninvasive accurate diagnosis and prediction of kidney transplant rejection
Science Translational Medicine, Mar 17, 2020; DOI: 10.1126/scitranslmed.aba2501
In vivo therapeutic efficacies and neural toxicity of F9170 in SHIV-infected rhesus macaques
Authors: Shan Su and Jing Xue
Date: Jun 15, 2020，Views：945
Original protocol used in research article：
An amphipathic peptide targeting the gp41 cytoplasmic tail kills HIV-1 virions and infected cells
Science Translational Medicine, Jun 2, 2020; DOI: 10.1126/scitranslmed.aaz2254
Characterization of nano-Ag@erythrosomes
Author: Xiao Han
Date: Jun 14, 2020，Views：1000
Original protocol used in research article：
Red blood cell–derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy
Science Advances, Oct 23, 2019; DOI: 10.1126/sciadv.aaw6870
In vivo distribution study
Author: Xiao Han
Date: Jun 14, 2020，Views：903
Original protocol used in research article：
Red blood cell–derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy
Science Advances, Oct 23, 2019; DOI: 10.1126/sciadv.aaw6870
Apoptosis assay
Authors: Lingzhi Zhao, Juanjuan Peng and Yanli Zhao
Date: Jun 11, 2020，Views：971
Original protocol used in research article：
MTH1 inhibitor amplifies the lethality of reactive oxygen species to tumor in photodynamic therapy
Science Advances, Mar 4, 2020; DOI: 10.1126/sciadv.aaz0575
Mitochondrial membrane potential measurements
Authors: Stefania Monterisi and Manuela Zaccolo
Date: Jun 9, 2020，Views：1091
Original protocol used in research article：
PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling
eLife, May 2, 2017; DOI: 10.7554/eLife.21374
IFT injection frequency distributions
Authors: Shane G McInally and Scott C Dawson
Date: Jun 4, 2020，Views：1075
Original protocol used in research article：
Length-dependent disassembly maintains four different flagellar lengths in Giardia
eLife, Dec 19, 2019; DOI: 10.7554/eLife.48694
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