Protocols shared by authors via RaP service
Journal
MBD7 ChIP
Authors: Dongming Li, Xuemei Chen and Julie A Law
Date: Aug 31, 2020，Views：945
Original protocol used in research article：
The MBD7 complex promotes expression of methylated transgenes without significantly altering their methylation status
eLife, Apr 28, 2017; DOI: 10.7554/eLife.19893
Biodegradation of PLGA-MPs
Authors: Xueguang Lu and Ana Jaklenec
Date: Aug 28, 2020，Views：947
Original protocol used in research article：
Engineered PLGA microparticles for long-term pulsatile release of STING agonist for cancer immunotherapy
Science Translational Medicine, Aug 11, 2020; DOI: 10.1126/scitranslmed.aaz6606
RT-LAMP assay
Authors: Viet Loan Dao Thi, Michael Knop, Simon Anders, Konrad Herbst, Matthias Meurer and Daniel Kirrmaier
Date: Aug 28, 2020，Views：1224
Original protocol used in research article：
A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Science Translational Medicine, Aug 12, 2020; DOI: 10.1126/scitranslmed.abc7075
S9.6 Slot blotting
Authors: Karlene A Cimprich and Madzia P Crossley
Date: Aug 27, 2020，Views：1656
Original protocol used in research article：
Co-transcriptional R-loops are the main cause of estrogen-induced DNA damage
eLife, Aug 23, 2016; DOI: 10.7554/eLife.17548
Measurement of transcriptional drift and drift-variance
Author: Michael Petrascheck
Date: Aug 27, 2020，Views：1028
Original protocol used in research article：
Suppression of transcriptional drift extends C. elegans lifespan by postponing the onset of mortality
eLife, Dec 1, 2015; DOI: 10.7554/eLife.08833
Recombinant oligonucleosomes
Authors: Fangfang Jiao and Rui Guo
Date: Aug 27, 2020，Views：820
Original protocol used in research article：
RACK7 recognizes H3.3G34R mutation to suppress expression of MHC class II complex components and their delivery pathway in pediatric glioblastoma
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba2113
PCR-RFLP of G1 and G2 mutations in the human Apolipoprotein-L1 (APOL1) gene
Author: Anneli Cooper
Date: Aug 25, 2020，Views：1398
Original protocol used in research article：
APOL1 renal risk variants have contrasting resistance and susceptibility associations with African trypanosomiasis
eLife, May 24, 2017; DOI: 10.7554/eLife.25461
ML algorithms and performance measures
Author: Juliana Bernardes
Date: Aug 25, 2020，Views：868
Original protocol used in research article：
Structure-specific DNA recombination sites: Design validation and machine learning–based refinement
Science Advances, Jul 23, 2020; DOI: 10.1126/sciadv.aay2922
Immunostaining
Author: Junhua Yang
Date: Aug 24, 2020，Views：953
Original protocol used in research article：
Astrocytes contribute to synapse elimination via type 2 inositol 1,4,5-trisphosphate receptor-dependent release of ATP
eLife, Apr 12, 2016; DOI: 10.7554/eLife.15043
Encapsulated Rapamycin Feeding Protocol
Authors: Matt Kaeberlein and Jonathan An
Date: Aug 24, 2020，Views：1526
Original protocol used in research article：
Rapamycin rejuvenates oral health in aging mice
eLife, Apr 28, 2020; DOI: 10.7554/eLife.54318
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