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CytofRUV vignette

MT Marie Trussart

Last updated date: Oct 19, 2020 Views: 974 Forks: 0

original An abbreviated version of this protocol was published in eLife in Sep, 2020
Removing unwanted variation with CytofRUV to integrate multiple CyTOF datasets
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CytofRUV is a computational algorithm which permits the integration of data across CyTOF batches. We provided a vignette Introduction_to_CytofRUV.Rmd on our github webpage that explains step by step how to load and normalise datasets and also how to visualise the diagnostic plots using the R-Shiny application before and after CytofRUV normalisation. 

We also provided an example of a set of data that included all .fcs, a metadata file a and panel file in the inst/extdata folder to run this example vignette. You can find this vignette here:


https://github.com/mtrussart/CytofRUV/blob/master/vignettes/Introduction_to_CytofRUV.Rmd



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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Trussart, M(2020). CytofRUV vignette. Bio-protocol Preprint. bio-protocol.org/prep552.
  2. Trussart, M., Teh, C. E., Tan, T., Leong, L., Gray, D. H. and Speed, T. P.(2020). Removing unwanted variation with CytofRUV to integrate multiple CyTOF datasets. eLife. DOI: 10.7554/eLife.59630

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