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Synthesis and characterization of CpG-PP, CpG-PM, and CpG-P-ss-M
Authors: Huile Gao and Lin Qin
Date: Sep 10, 2020，Views：977
Original protocol used in research article：
A tumor-to-lymph procedure navigated versatile gel system for combinatorial therapy against tumor recurrence and metastasis
Science Advances, Sep 3, 2020; DOI: 10.1126/sciadv.abb3116
Quantitative RT-PCR analysis
Author: Katherine A Donovan
Date: Sep 10, 2020，Views：1011
Original protocol used in research article：
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
eLife, Aug 1, 2018; DOI: 10.7554/eLife.38430
Detection and quantitation of NAD+- and NADH-capped RNA in vivo: hybridization with a radiolabeled oligodeoxyribonucleotide probe
Author: Jeremy G Bird
Date: Sep 10, 2020，Views：974
Original protocol used in research article：
Highly efficient 5' capping of mitochondrial RNA with NAD+ and NADH by yeast and human mitochondrial RNA polymerase
eLife, Dec 12, 2018; DOI: 10.7554/eLife.42179
Histology and molecular phenotyping
Author: Magda Spella
Date: Sep 9, 2020，Views：975
Original protocol used in research article：
Club cells form lung adenocarcinomas and maintain the alveoli of adult mice
eLife, May 29, 2019; DOI: 10.7554/eLife.45571
Sample preparation - Elemental mapping
Author: Gawain McColl
Date: Sep 9, 2020，Views：929
Original protocol used in research article：
Changes in ferrous iron and glutathione promote ferroptosis and frailty in aging Caenorhabditis elegans
eLife, Jul 21, 2020; DOI: 10.7554/eLife.56580
Preparation of brain samples for immunofluorescence, immunoblot, and nucleic acid analysis
Author: Bradlee L. Heckmann
Date: Sep 8, 2020，Views：867
Original protocol used in research article：
Noncanonical function of an autophagy protein prevents spontaneous Alzheimer’s disease
Science Advances, Aug 13, 2020; DOI: 10.1126/sciadv.abb9036
Thermostability shift assay and library screening
Author: Edmund R.S. Kunji
Date: Sep 7, 2020，Views：960
Original protocol used in research article：
Screening of candidate substrates and coupling ions of transporters by thermostability shift assays
eLife, Oct 15, 2018; DOI: 10.7554/eLife.38821
In vitro 2d matrigel migration and protrusion assay
Authors: Monte M Winslow, Julien Sage and Dian Yang
Date: Sep 6, 2020，Views：1054
Original protocol used in research article：
Axon-like protrusions promote small cell lung cancer migration and metastasis
eLife, Dec 13, 2019; DOI: 10.7554/eLife.50616
Anti-aggregation assays
Author: Tomohide Saio
Date: Sep 4, 2020，Views：988
Original protocol used in research article：
Oligomerization of a molecular chaperone modulates its activity
eLife, May 1, 2018; DOI: 10.7554/eLife.35731
WorMotel preparation
Author: Christopher Fang-Yen
Date: Sep 4, 2020，Views：1350
Original protocol used in research article：
Longitudinal imaging of Caenorhabditis elegans in a microfabricated device reveals variation in behavioral decline during aging
eLife, May 31, 2017; DOI: 10.7554/eLife.26652
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