Protocols shared by authors via RaP service
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Detail Immunostaining Protocol for Pax6 mouse eye samples
Author: Ali R. Djalilian
Date: Dec 15, 2020，Views：769
Original protocol used in research article：
Gene dosage manipulation alleviates manifestations of hereditary PAX6 haploinsufficiency in mice
Science Translational Medicine, Dec 9, 2020; DOI: 10.1126/scitranslmed.aaz4894
Cell culture and reagents
Author: Melanie Girard
Date: Dec 15, 2020，Views：6067
Original protocol used in research article：
Type I interferons drive the maturation of human DC3s with a distinct costimulatory profile characterized by high GITRL
Science Immunology, Nov 12, 2020; DOI: 10.1126/sciimmunol.abe0347
Identification of species-specific expressed genes
Author: Jie Wang
Date: Dec 12, 2020，Views：6225
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
Pre-processing of fiber photometry signal
Author: Richard B Crouse
Date: Dec 11, 2020，Views：1012
Original protocol used in research article：
Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency
eLife, Sep 18, 2020; DOI: 10.7554/eLife.57335
RNA constructs and in vitro transcription.
Author: Katharina Reinhard
Date: Dec 10, 2020，Views：977
Original protocol used in research article：
An RNA vaccine drives expansion and efficacy of claudin-CAR-T cells against solid tumors
Science, Jan 1, 2020; DOI: 10.1126/science.aay5967
STED imaging
Author: Monica M Sousa
Date: Dec 10, 2020，Views：847
Original protocol used in research article：
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction
eLife, Mar 20, 2020; DOI: 10.7554/eLife.55471
Composite agarose-polyacrylamide gel electrophoresis (Composite AgPAGE)
Authors: Kirk Bergstrom, Xindi Shan and Lijun Xia
Date: Dec 9, 2020，Views：974
Original protocol used in research article：
Proximal colon–derived O-glycosylated mucus encapsulates and modulates the microbiota
Science, Oct 23, 2020; DOI: 10.1126/science.aay7367
Adoptive cell transfer (ACT) and RNA-LPX treatment
Author: Katharina Reinhard
Date: Dec 9, 2020，Views：1026
Original protocol used in research article：
An RNA vaccine drives expansion and efficacy of claudin-CAR-T cells against solid tumors
Science, Jan 1, 2020; DOI: 10.1126/science.aay5967
Ex vivo live imaging
Authors: Anamika Sharma and Gaiti Hasan
Date: Dec 9, 2020，Views：1136
Original protocol used in research article：
Modulation of flight and feeding behaviours requires presynaptic IP3Rs in dopaminergic neurons
eLife, Nov 6, 2020; DOI: 10.7554/eLife.62297
Bacterial flow cytometry (BUGFlow)
Author: Anne-Katrin Pröbstel
Date: Dec 9, 2020，Views：1110
Original protocol used in research article：
Gut microbiota–specific IgA+ B cells traffic to the CNS in active multiple sclerosis
Science Immunology, Nov 19, 2020; DOI: 10.1126/sciimmunol.abc7191
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