Protocols shared by authors via RaP service
Journal
Oil-Red-O staining to determine the amount of stored triglyceride in Caenorhabditis elegans
Authors: Avijit Mallick and Bhagwati P Gupta
Date: Mar 15, 2021，Views：832
Original protocol used in research article：
Vitellogenin-2 acts downstream of PRY-1/Axin to regulate lipids and lifespan in C. elegans
microPublication Biology, Jul 21, 2020; DOI: 10.17912/micropub.biology.000281
Calculation of VISTA module score
Author: Yanding Zhao
Date: Mar 15, 2021，Views：823
Original protocol used in research article：
VISTA is a checkpoint regulator for naïve T cell quiescence and peripheral tolerance
Science, Jan 16, 2020; DOI: 10.1126/science.aay0524
Virus neutralization assay
Authors: Yasumasa Iwatani and Hiroshi Murakami
Date: Mar 15, 2021，Views：959
Original protocol used in research article：
Antibody-like proteins that capture and neutralize SARS-CoV-2
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3916
Computational methods
Authors: Lizhi Liu and Sheng Chen
Date: Mar 15, 2021，Views：817
Original protocol used in research article：
Mechanical penetration of β-lactam–resistant Gram-negative bacteria by programmable nanowires
Science Advances, Jul 2, 2020; DOI: 10.1126/sciadv.abb9593
Headloop PCR
Author: Gareth Thomas Powell
Date: Mar 15, 2021，Views：2277
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
ChIP-seq
Author: Wen Tang
Date: Mar 15, 2021，Views：933
Original protocol used in research article：
ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL
eLife, Feb 17, 2020; DOI: 10.7554/eLife.52091
De novo protein design
Author: Anastassia A. Vorobieva
Date: Mar 15, 2021，Views：885
Original protocol used in research article：
De novo design of transmembrane β barrels
Science, Feb 18, 2021; DOI: 10.1126/science.abc8182
Organoid experiments culture & seeding
Authors: Wouter R. Karthaus, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：997
Original protocol used in research article：
Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267
Prostate isolation and enzymatic digestion
Authors: Wouter R. Karthaus, Matan Hofree, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：969
Original protocol used in research article：
Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267
Single-cell dissociation and RNA sequencing
Author: Michael L Wallace
Date: Mar 11, 2021，Views：858
Original protocol used in research article：
Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.