The immunocytochemistry/immunohistochemistry with synaptic puncta analysis is outlined in detail in our previously published work in JoVe, freely available via PMC at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159596/.
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How to cite:
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
Risher, W and Eroglu, C(2021). Immunohistochemistry and synaptic puncta analysis. Bio-protocol Preprint. bio-protocol.org/prep1030.
Risher, W. C., Patel, S., Kim, I. H., Uezu, A., Bhagat, S., Wilton, D. K., Pilaz, L., Singh Alvarado, J., Calhan, O. Y., Silver, D. L., Stevens, B., Calakos, N., Soderling, S. H. and Eroglu, C.(2014). Astrocytes refine cortical connectivity at dendritic spines. eLife. DOI: 10.7554/eLife.04047
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