癌症生物学

分类

    现刊
    Measurement of Cell Intrinsic TGF-β Activation Mediated by the Integrin αvβ8
    整合素αvβ8介导的细胞固有TGF-β活化测定
    作者:Robert Ian Seed and Stephen Lloyd Nishimura日期:04/20/2022,浏览量:1062,Q&A: 0
    [Abstract]

    Transforming growth factor beta (TGF-β) is a multi-functional cytokine that plays a significant role in multiple diseases, including fibrosis and tumor progression. Whilst the biologic effects of TGF-β are well characterized, it is unclear how TGF-β signaling is regulated to impart specific responses within certain cell types. One

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    Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids
    用于人结直肠上皮类器官和类肿瘤的低粘度基质悬浮培养
    作者:Tao Tan, Yumiko Hirokawa, Jordan Clarke, Anuratha Sakthianandeswaren and Oliver M. Sieber日期:04/20/2022,浏览量:804,Q&A: 0
    [Abstract]

    Three-dimensional culture of human normal colorectal epithelium and cancer tissue as organoids and tumoroids has transformed the study of diseases of the large intestine. A widely used strategy for generating patient-derived colorectal organoids and tumoroids involves embedding cells in domes of extracellular matrix (ECM). Despite its success,

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    Conditional Human BRD4 Knock-In Transgenic Mouse Genotyping and Protein Isoform Detection
    条件性人 BRD4 敲入转基因小鼠基因分型和蛋白质异构体检测
    作者:Michael Paul Lewis, Shwu-Yuan Wu and Cheng-Ming Chiang日期:04/05/2022,浏览量:410,Q&A: 0
    [Abstract]

    Bromodomain-containing protein 4 (BRD4) is an acetyl-lysine reader protein and transcriptional regulator implicated in chromatin dynamics and cancer development. Several BRD4 isoforms have been detected in humans with the long isoform (BRD4-L, aa 1-1,362) playing a tumor-suppressive role and a major short isoform (BRD4-S, aa 1-722) having

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    Image-based Quantification of Macropinocytosis Using Dextran Uptake into Cultured Cells
    基于图像定量使用葡聚糖摄取到培养细胞中的巨胞饮
    作者:Anh H. Le and Laura M. Machesky日期:04/05/2022,浏览量:510,Q&A: 0
    [Abstract]

    Macropinocytosis is an evolutionarily conserved process, which is characterized by the formation of membrane ruffles and the uptake of extracellular fluid. We recently demonstrated a role for CYFIP-related Rac1 Interactor (CYRI) proteins in macropinocytosis. High-molecular weight dextran (70kDa or higher) has generally been used as a marker for

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    Single-cell Damagenome Profiling by Linear Copying and Splitting based Whole Genome Amplification (LCS-WGA)
    基于线性复制和分裂的全基因组扩增法(LCS-WGA)分析单细胞损伤组
    作者:Yichi Niu, Qiangyuan Zhu and Chenghang Zong日期:03/20/2022,浏览量:485,Q&A: 0
    [Abstract]

    Spontaneous DNA damage frequently occurs on the human genome, and it could alter gene expression by inducing mutagenesis or epigenetic changes. Therefore, it is highly desired to profile DNA damage distribution on the human genome and identify the genes that are prone to DNA damage. Here, we present a novel single-cell whole-genome amplification

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    3D-Structured Illumination Microscopy of Centrosomes in Human Cell Lines
    人类细胞系中心体的 3D 结构照明显微镜
    作者:Kari-Anne M. Frikstad, Kay O. Schink, Sania Gilani, Lotte B. Pedersen and Sebastian Patzke日期:03/20/2022,浏览量:704,Q&A: 0
    [Abstract]

    The centrosome is the main microtubule-organizing center of animal cells, and is composed of two barrel-shaped microtubule-based centrioles embedded in protein dense pericentriolar material. Compositional and architectural re-organization of the centrosome drives its duplication, and enables its microtubule-organizing activity and capability to

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    Three-dimensional Models of the Nasopharynx for the Study of Epstein-Barr Virus Infection
    研究EB病毒感染的鼻咽三维模型
    [Abstract]

    The ubiquitous and cancer-associated Epstein-Barr virus (EBV) is associated with nearly all cases of nasopharyngeal carcinoma (NPC). Nasopharyngeal tissue is comprised of both pseudostratified and stratified epithelium, which are modeled in three-dimensional (3-D) cell culture. The cellular origin of EBV-associated NPC is as yet unknown, but both

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    Small Molecule Screening of Primary Human Acute Myeloid Leukemia Using Co-culture and Multiplexed FACS Analysis
    使用共培养和多重 FACS 分析对原发性人类急性髓细胞白血病进行小分子筛选
    作者:Aurelie Baudet, Simon Hultmark, Fredrik Ek and Mattias Magnusson日期:03/20/2022,浏览量:440,Q&A: 0
    [Abstract]

    Ex vivo culture of primary acute myeloid leukemia (AML) cells is notoriously difficult due to spontaneous differentiation and cell death, which hinders mechanistic and translational studies. To overcome this bottleneck, we have implemented a co-culture system, where the OP9-M2 stromal cells support the growth, but most notably limit the

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    Whole-genome Methylation Analysis of APOBEC Enzyme-converted DNA (~5 kb) by Nanopore Sequencing
    通过纳米孔测序对 APOBEC 酶转化 DNA (~5 kb) 进行全基因组甲基化分析
    作者:Suzuko Zaha, Yoshitaka Sakamoto, Satoi Nagasawa, Sumio Sugano, Ayako Suzuki, Yutaka Suzuki and Masahide Seki日期:03/05/2022,浏览量:1678,Q&A: 0
    [Abstract]

    In recent years, DNA methylation research has been accelerated by the advent of nanopore sequencers. However, read length has been limited by the constraints of base conversion using the bisulfite method, making analysis of chromatin content difficult. The read length of the previous method combining bisulfite conversion and long-read sequencing

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    Labeling Endogenous Proteins Using CRISPR-mediated Insertion of Exon (CRISPIE)
    使用 CRISPR 介导的外显子插入 (CRISPIE) 标记内源性蛋白质
    作者:Evan A. Wilson, Tianyi Mao and Haining Zhong日期:03/05/2022,浏览量:1891,Q&A: 0
    [Abstract]

    The CRISPR/Cas9 technology has transformed our ability to edit eukaryotic genomes. Despite this breakthrough, it remains challenging to precisely knock-in large DNA sequences, such as those encoding a fluorescent protein, for labeling or modifying a target protein in post-mitotic cells. Previous efforts focusing on sequence insertion to the

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