癌症生物学

分类

    现刊
    Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells
    在SKBR3细胞中内源性HER2和HER3受体的聚类分析
    The Human Epidermal Growth Factor Receptor (HER) family of receptor tyrosine kinases consists of four, single pass, transmembrane receptor homologs (HER1-4) that act to regulate many critical processes in normal and tumor cells. HER2 is ...
    Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells
    在SKBR3细胞中内源性HER2和HER3受体的聚类分析
    [Abstract] The Human Epidermal Growth Factor Receptor (HER) family of receptor tyrosine kinases consists of four, single pass, transmembrane receptor homologs (HER1-4) that act to regulate many critical processes in normal and tumor cells. HER2 is overexpressed in many tumors, and the deregulated proliferation of cancerous cells is driven by cooperation with ...
    A Functionally Robust Phenotypic Screen that Identifies Drug Resistance-associated Genes Using 3D Cell Culture
    利用3D细胞培养鉴定耐药相关基因的一种功能性稳健表型筛选
    作者:Sun-Young Lee and Mina J. Bissell日期:11/20/2018,浏览量:463,Q&A: 0
    [Abstract] Drug resistance is a major obstacle in cancer treatment: A case in point is the failure of cancer patients to respond to tyrosine kinase inhibitors (TKI) of EGFR, a receptor that is highly expressed in many cancers. Identification of the targets and delineation of mechanisms of drug resistance remain major challenges. Traditional pharmacological ...
    In-vivo gp100-specific Cytotoxic CD8+ T Cell Killing Assay
    体内gp100特异的细胞毒CD8+ T 细胞杀伤实验
    作者:Mahdia Benkhoucha, Nicolas Molnarfi, Elodie Belnoue, Madiha Derouazi and Patrice H Lalive日期:11/20/2018,浏览量:358,Q&A: 0
    [Abstract] Cytotoxic CD8+ T lymphocytes (CTLs) represent a crucial component of the adaptive immune system and play a prominent role in the anti-tumor immune responses of both mice and humans.

    Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived ...
    Ex vivo Culture and Lentiviral Transduction of Benign Prostatic Hyperplasia (BPH) Samples
    良性前列腺增生(BPH)样本的离体培养和慢病毒转染
    作者:Urszula Lucja McClurg, Stuart R McCracken, Lisa Butler, Karl T Riabowol and Olivier Binda日期:11/05/2018,浏览量:193,Q&A: 0
    [Abstract] To assess oncogenic potential, classical transformation assays are based on cell line models. However, cell line based models do not reflect the complexity of human tissues. We thus developed an inducible expression system for gene expression in ex vivo human tissues, which maintain native tissue architecture, such as epithelia and ...
    Protocol for in situ Proximity Ligation Assay (PLA) and Microscopy Analysis of Epidermal Growth Factor Receptor (EGFR) Homodimerization
    原位邻位连接检测和显微镜分析表皮生长因子的同源二聚化的实验方法
    作者:Keiichi Ota and Taishi Harada日期:11/05/2018,浏览量:328,Q&A: 0
    [Abstract] Oncogenic drivers play central roles in tumorigenesis as well as in tumor cell survival and proliferation. Mutations of the epidermal growth factor receptor gene (EGFR) that result in constitutive activation of the receptor tyrosine kinase have been identified as oncogenic drivers in a subset of non-small cell lung cancer (NSCLC). ...
    Detection of Cell Death in Planarians
    真涡虫细胞死亡的检测
    作者:Nídia de Sousa and Teresa Adell日期:10/05/2018,浏览量:475,Q&A: 0
    [Abstract] Planarians are freshwater flatworms, well known for their ability to regenerate a complete organism from any piece of their body. Furthermore, planarians are constantly growing and degrowing throughout their lives, maintaining a functional and proportioned body. These properties rely on the presence of a population of adult stem cells and on the ...
    Qualitative in vivo Bioluminescence Imaging
    体内定性生物发光成像技术
    作者:Devbarna Sinha, Zalitha Pieterse and Pritinder Kaur日期:09/20/2018,浏览量:607,Q&A: 0
    [Abstract] Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the ...
    Zebrafish Embryo Xenograft and Metastasis Assay
    斑马鱼胚胎植入和转移
    作者:Ilkka Paatero, Sanni Alve, Silvia Gramolelli, Johanna Ivaska and Päivi M. Ojala日期:09/20/2018,浏览量:659,Q&A: 0
    [Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...
    Cell Synchronization by Double Thymidine Block
    用胸腺嘧啶核苷双阻断法同步细胞
    作者:Guo Chen and Xingming Deng日期:09/05/2018,浏览量:2542,Q&A: 1
    [Abstract] Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at ...
    Microarray, IPA and GSEA Analysis in Mice Models
    关于小鼠模型的微阵列,IPA和GSEA分析
    作者:Stephanie N. Oprescu, Katharine A. Horzmann, Feng Yue, Jennifer L. Freeman and Shihuan Kuang日期:09/05/2018,浏览量:581,Q&A: 0
    [Abstract] This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms underlying biological differences across two samples and thus can be applied to interrogate a variety ...