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癌症生物学

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    现刊
    FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
    基于FACS利用EGF配体从新鲜人组织中分离神经和胶质瘤干细胞群
    作者:Jessica Tome-Garcia, Fiona Doetsch and Nadejda M. Tsankova日期:12/20/2017,浏览量:522,Q&A: 0
    [Abstract] Direct isolation of human neural and glioma stem cells from fresh tissues permits their biological study without prior culture and may capture novel aspects of their molecular phenotype in their native state. Recently, we demonstrated the ability to prospectively isolate stem cell populations from fresh human germinal matrix and glioblastoma ...
    In vivo Leukemogenesis Model Using Retrovirus Transduction
    采用逆转录病毒转导的体内白血病生成模型
    作者:Hiroshi Okuda and Akihiko Yokoyama日期:12/05/2017,浏览量:314,Q&A: 0
    [Abstract] Various genetic alterations such as chromosomal translocation cause leukemia. For examples, gene rearrangements of the mixed-lineage leukemia (MLL) gene generate MLL fusion genes, whose products are potent oncogenic drivers in acute leukemia. To better understand the mechanism of disease onset, several murine leukemia models ...
    Myeloid Progenitor Transformation Assay
    髓系祖细胞转化试验
    作者:Hiroshi Okuda and Akihiko Yokoyama日期:12/05/2017,浏览量:347,Q&A: 0
    [Abstract] Numerous oncogenes have been identified to cause leukemia. For example, chromosomal translocation generates various fusion genes of the mixed-lineage leukemia (MLL) gene and a partner gene in leukemia, whose gene products transform primary myeloid progenitors into an immortalized state. To characterize the transforming ability of leukemic ...
    An Affinity-directed Protein Missile (AdPROM) System for Targeted Destruction of Endogenous Proteins
    靶向破坏内源蛋白的亲和力定向蛋白导弹(AdPROM)系统
    作者:Thomas J Macartney, Gopal P Sapkota and Luke J Fulcher日期:11/20/2017,浏览量:585,Q&A: 0
    [Abstract] We recently reported an Affinity-directed PROtein Missile (AdPROM) system for the targeted proteolysis of endogenous proteins of interest (POI) (Fulcher et al., 2016 and 2017). AdPROM consists of the Von Hippel Lindau (VHL) protein, a Cullin 2 E3 ligase substrate receptor (Bosu and Kipreos, 2008), conjugated to a high affinity polypeptide ...
    In vitro NLK Kinase Assay
    体外NLK激酶测定
    作者:Sungho Moon, Jiyoung Kim and Eek‐hoon Jho日期:11/05/2017,浏览量:550,Q&A: 0
    [Abstract] This protocol provides step by step instructions to perform an in vitro kinase assay for nemo-like kinase. In addition, this protocol also describes an efficient method using mild lysis buffer for expression and purification of Glutathione S-transferase (GST) fusion proteins.
    Xenograft Mouse Model of Human Uveal Melanoma
    人葡萄膜黑色素瘤异种移植小鼠模型
    作者:Yao Chen, Xiao Liu, Ling Gao and Yongqing Liu日期:11/05/2017,浏览量:563,Q&A: 0
    [Abstract] Uveal melanoma (UM) is a malignant intraocular tumor in adults. Metastasis develops in almost half of the patients and over 90% of the metastases are in the liver. With the advances in molecular targeting therapy for melanoma, a proper metastasis animal model is of increasing importance for testing the accuracy and effectiveness of systemic ...
    Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells
    用于监测蒽环类药物进入哺乳动物细胞的摄取分析
    作者:Nicolas Brosseau, Emil Andreev and Dindial Ramotar日期:09/20/2017,浏览量:754,Q&A: 0
    [Abstract] Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be exploited to identify components that are involved in the uptake of anthracyclines.
    Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures
    在细胞培养中建立基于多重图像的自噬RNAi筛选方法
    作者:Jennifer Jung and Christian Behrends日期:09/05/2017,浏览量:1046,Q&A: 0
    [Abstract] Autophagy is a recycling pathway, in which intracellular cargoes including protein aggregates and bacteria are engulfed by autophagosomes and subsequently degraded after fusion with lysosomes. Dysregulation of this process is involved in several human diseases such as cancer or neurodegeneration. Hence, advancing our understanding of how autophagy ...
    Isolation and Separation of Epithelial CD34+ Cancer Stem Cells from Tgfbr2-deficient Squamous Cell Carcinoma
    从Tgfbr2缺乏鳞状细胞癌分离上皮CD34+癌干细胞
    作者:Heather A. McCauley and Géraldine Guasch日期:09/05/2017,浏览量:1044,Q&A: 0
    [Abstract] Most epithelial tumors have been shown to contain cancer stem cells that are potentially the driving force in tumor progression and metastasis (Kreso and Dick, 2014; Nassar and Blanpain, 2016). To study these cells in depth, cell isolation strategies relying on cell surface markers or fluorescent reporters are essential, and the isolation ...
    GFP-Grb2 Translocation Assay Using High-content Imaging to Screen for Modulators of EGFR-signaling
    通过高含量成像观测GFP-Grb2易位筛查EGFR信号转导调控因子
    作者:Julia Petschnigg and Robin Ketteler日期:09/05/2017,浏览量:935,Q&A: 0
    [Abstract] High-content screening is a useful tool to understand complex cellular processes and to identify genes, proteins or small molecule compounds that modulate such pathways. High-content assays monitor the function of a protein or pathway by visualizing a change in an image-based readout, such as a change in the localization of a reporter protein. ...