微生物学

分类

    现刊
    Enterovirus Competition Assay to Assess Replication Fitness
    用于检测复制适应性的肠道病毒竞争实验
    作者:Valeria Lulla and Andrew E. Firth日期:05/20/2019,浏览量:133,Q&A: 0
    In virology the difference between the fitness of two viruses can be determined by using various methods, such as virus titer, growth curve analysis, measurement of virus infectivity, analysis of produced RNA copies and viral protein production. ...
    TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4+ T Cells
    TZA, 一种基于敏感报告因子细胞学检测方法, 可用于准确快速量化静息CD4+ T细胞中可诱导的且复制能力强的潜伏HV-1
    作者:Anwesha Sanyal, Vatsala S. Rangachar and Phalguni Gupta日期:05/20/2019,浏览量:168,Q&A: 0
    The latent HIV-1 viral reservoir in resting CD4+ (rCD4+) T cells represents a major barrier to an HIV-1 cure. There is an ongoing effort to identify therapeutic approaches that will eliminate or reduce the size of this ...
    Purification and Proteomic Analysis of Alphavirus Particles from Sindbis Virus Grown in Mammalian and Insect Cells
    哺乳动物和昆虫细胞中的辛德比斯病毒的α病毒颗粒的纯化和蛋白质组分析
    作者:Raquel Hernandez, Trevor Glaros, Gabrielle Rizzo and Davis F. Ferreira日期:05/20/2019,浏览量:126,Q&A: 0
    Current mass spectrometry (MS) methods and new instrumentation now allow for more accurate identification of proteins in low abundance than previous protein fractionation and identification methods. It was of interest if this method could serve to ...
    Biofilm Formation Assay in Pseudomonas syringae
    丁香假单胞菌的生物膜形成实验
    作者:Xiaolong Shao, Yingpeng Xie, Yingchao Zhang and Xin Deng日期:05/20/2019,浏览量:197,Q&A: 0
    Pseudomonas syringae is a model plant pathogen that infects more than 50 plant species worldwide, thus leading to significant yield loss. Pseudomonas biofilm always adheres to the surfaces of medical devices or host cells, thereby ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:420,Q&A: 0
    The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial ...
    Assembly of a Custom-made Device to Study Spreading Patterns of Pseudomonas putida Biofilms
    用于恶臭假单胞菌生物膜扩散模式研究的定制设备的组装
    作者:David R. Espeso, Esteban Martínez-García and Víctor de Lorenzo日期:05/20/2019,浏览量:172,Q&A: 0
    Biofilms are bacterial communities in the shape of exopolysaccharide matrix-encased aggregates attached onto interphases able to resist environmental aggressions. The development of bacteria in the shape of biofilms deeply affects the performance of ...
    Enterovirus Competition Assay to Assess Replication Fitness
    用于检测复制适应性的肠道病毒竞争实验
    作者:Valeria Lulla and Andrew E. Firth日期:05/20/2019,浏览量:133,Q&A: 0
    [Abstract] In virology the difference between the fitness of two viruses can be determined by using various methods, such as virus titer, growth curve analysis, measurement of virus infectivity, analysis of produced RNA copies and viral protein production. However, for closely performing viruses, it is often very hard to distinguish the differences. In vitro ...
    TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4+ T Cells
    TZA, 一种基于敏感报告因子细胞学检测方法, 可用于准确快速量化静息CD4+ T细胞中可诱导的且复制能力强的潜伏HV-1
    作者:Anwesha Sanyal, Vatsala S. Rangachar and Phalguni Gupta日期:05/20/2019,浏览量:168,Q&A: 0
    [Abstract] The latent HIV-1 viral reservoir in resting CD4+ (rCD4+) T cells represents a major barrier to an HIV-1 cure. There is an ongoing effort to identify therapeutic approaches that will eliminate or reduce the size of this reservoir. However, clinical investigators lack an assay to determine whether or not a decrease in the ...
    Purification and Proteomic Analysis of Alphavirus Particles from Sindbis Virus Grown in Mammalian and Insect Cells
    哺乳动物和昆虫细胞中的辛德比斯病毒的α病毒颗粒的纯化和蛋白质组分析
    作者:Raquel Hernandez, Trevor Glaros, Gabrielle Rizzo and Davis F. Ferreira日期:05/20/2019,浏览量:126,Q&A: 0
    [Abstract] Current mass spectrometry (MS) methods and new instrumentation now allow for more accurate identification of proteins in low abundance than previous protein fractionation and identification methods. It was of interest if this method could serve to define the virus proteome of a membrane-containing virus. To evaluate the efficacy of mass spec to ...
    Biofilm Formation Assay in Pseudomonas syringae
    丁香假单胞菌的生物膜形成实验
    作者:Xiaolong Shao, Yingpeng Xie, Yingchao Zhang and Xin Deng日期:05/20/2019,浏览量:197,Q&A: 0
    [Abstract] Pseudomonas syringae is a model plant pathogen that infects more than 50 plant species worldwide, thus leading to significant yield loss. Pseudomonas biofilm always adheres to the surfaces of medical devices or host cells, thereby contributing to infection. Biofilm formation can be visualized on numerous matrixes, including ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:420,Q&A: 0
    [Abstract] The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas ...
    Assembly of a Custom-made Device to Study Spreading Patterns of Pseudomonas putida Biofilms
    用于恶臭假单胞菌生物膜扩散模式研究的定制设备的组装
    作者:David R. Espeso, Esteban Martínez-García and Víctor de Lorenzo日期:05/20/2019,浏览量:172,Q&A: 0
    [Abstract] Biofilms are bacterial communities in the shape of exopolysaccharide matrix-encased aggregates attached onto interphases able to resist environmental aggressions. The development of bacteria in the shape of biofilms deeply affects the performance of many industrial processes which work with fluidic systems, where bacteria may settle and prosper. ...
    Analysis of Indole-3-acetic Acid (IAA) Production in Klebsiella by LC-MS/MS and the Salkowski Method
    利用液相色谱-串联质谱法和萨尔科夫斯基氏法分析克雷伯菌中吲哚乙酸的产生
    作者:Shraddha Gang, Sheetal Sharma, Meenu Saraf, Martin Buck and Jörg Schumacher日期:05/05/2019,浏览量:292,Q&A: 0
    [Abstract] Many rhizobacteria isolated from plant rhizosphere produce various phytohormones in the form of secondary metabolites, the most common of which is Indole-3-acetic acid (IAA). Here, we detail analytical protocols of IAA detection and quantification, in vitro and in situ, as recently applied to Klebsiella SGM 81, a ...
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    根相互作用系统的示踪实验和质量控制
    作者:Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni日期:04/20/2019,浏览量:650,Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Unbiased Screening of Activated Receptor Tyrosine Kinases (RTKs) in Tumor Extracts Using a Mouse Phospho-RTK Array Kit
    利用小鼠Phospho-RTK Array试剂盒无偏筛选肿瘤提取物中激活受体酪氨酸激酶
    作者:Julian Naipauer, Lucas E. Cavallin and Enrique A. Mesri日期:04/20/2019,浏览量:375,Q&A: 0
    [Abstract] Kaposi’s sarcoma (KS) herpesvirus (KSHV) is a virus that causes KS, an angiogenic AIDS-associated spindle-cell neoplasm, by activating host oncogenic signaling cascades through autocrine and paracrine mechanisms. Many host signaling cascades co-opted by KSHV including PI3K/AKT/mTORC, NFkB and Notch are critical for cell-specific mechanisms of ...
    Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae
    在单细胞红藻 Cyanidioschyzon merolae中利用URA5.3选择标记对染色体基因进行多重修饰
    作者:Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka日期:04/05/2019,浏览量:372,Q&A: 0
    [Abstract] The unicellular red alga Cyanidioschyzon merolae has been used as a eukaryotic photosynthetic model for various basic and applied studies. Although the nuclear genome of C. merolae can be modified by homologous recombination with exogenously introduced DNA, it has been difficult to modify multiple chromosome loci within the same ...