分子生物学

分类

    现刊
    Novel Protein-oligonucleotide Conjugation Method Involving a High-affinity Capture HaloTag
    一种高亲和力捕获卤代烷脱氢酶标记的蛋白质寡核苷酸偶联新方法
    作者:Junshi Yazaki日期:09/20/2020,浏览量:96,Q&A: 0
    [Abstract] Highly sensitive quantitative protein profiling can play a key role in the early diagnosis of diseases, such as autoimmune diseases and cancer. We developed a modified protein-oligonucleotide conjugation method termed HaloTag-mediated barcoding, for quantifying protein molecules at a higher sensitivity than conventional protein quantification ...
    Quantification of the Surface Expression of G Protein-coupled Receptors Using Intact Live-cell Radioligand Binding Assays
    利用完整活细胞放射配体结合分析定量G蛋白偶联受体的表面表达
    作者:Xin Xu and Guangyu Wu日期:09/20/2020,浏览量:100,Q&A: 0
    [Abstract] G protein-coupled receptors (GPCRs) are the most structurally diverse family of signaling proteins and regulate a variety of cell function. For most GPCRs, the cell surface is their functional destination where they are able to respond a wide range of extracellular stimuli, leading to the activation of intracellular signal transduction cascades. ...
    A Workflow for Ultra-rapid Analysis of Histone Post-translational Modifications with Direct-injection Mass Spectrometry
    使用直接进样质谱对组蛋白翻译后修饰进行超快速分析的工作流程
    作者:Natarajan V. Bhanu, Simone Sidoli and Benjamin A Garcia日期:09/20/2020,浏览量:99,Q&A: 0
    [Abstract] Chromatin modifications, like histone post translational modifications (PTMs), are critical for tuning gene expression and many other aspects of cell phenotype. Liquid chromatography coupled to mass spectrometry (LC-MS) has become the most suitable method to analyze histones and histone PTMs in a large-scale manner. Selected histone PTMs have ...
    Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome
    心肾综合征新型小鼠模型中肾脏a-SMA,胶原1和胶原3的免疫组化研究
    [Abstract] Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease (ESRD) population compared to the population as a whole thus necessitating the need for improved ...
    BRIDGE: An Open Platform for Reproducible Protein-Ligand Simulations and Free Energy of Binding Calculations
    BRIDGE:一个可重复的蛋白质配体模拟和结合自由能计算的开放平台
    作者:Tharindu Senapathi, Christopher B. Barnett and Kevin J. Naidoo日期:09/05/2020,浏览量:788,Q&A: 0
    [Abstract] Protein-ligand binding prediction is central to the drug-discovery process. This often follows an analysis of genomics data for protein targets and then protein structure discovery. However, the complexity of performing reproducible protein conformational analysis and ligand binding calculations, using vetted methods and protocols can be a ...
    Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in Nicotiana benthamiana
    转基因烟草瞬时表达后蛋白质丰度的比例计量测定
    作者:Aashima Khosla and David C. Nelson日期:09/05/2020,浏览量:517,Q&A: 0
    [Abstract] Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein fused to a second reporter. Both fusion proteins are encoded on a single transcript but are ...
    Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues
    存在不良成核核苷酸类似物的微管种晶组装
    作者:Siou Ku, Claire Heichette, Laurence Duchesne and Denis Chrétien日期:08/20/2020,浏览量:472,Q&A: 0
    [Abstract] Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor microtubule nucleators. Usually, microtubule assembly is seeded by guanylyl-(α, ...
    High-level Production of Recombinant Membrane Proteins Using the Engineered Escherichia coli Strains SuptoxD and SuptoxR
    利用工程大肠杆菌SuptoxD和SuptoxR高效生产重组膜蛋白
    作者:Myrsini Michou, Dafni C. Delivoria and Georgios Skretas日期:08/05/2020,浏览量:716,Q&A: 0
    [Abstract] We have previously described the development of two specialized Escherichia coli strains for high-level recombinant membrane protein (MP) production. These engineered strains, termed SuptoxD and SuptoxR, are capable of suppressing the cytotoxicity caused by MP overexpression and of producing greatly enhanced MP yields. Here, we present a ...
    A Method for User-defined Mutagenesis by Integrating Oligo Pool Synthesis Technology with Nicking Mutagenesis
    一种集成引物池合成技术和切口诱变的用户自定义突变方法
    作者:Paul J. Steiner, Zachary T Baumer and Timothy A. Whitehead日期:08/05/2020,浏览量:822,Q&A: 0
    [Abstract] Saturation mutagenesis is a fundamental enabling technology for protein engineering and epitope mapping. Nicking mutagenesis (NM) allows the user to rapidly construct libraries of all possible single mutations in a target protein sequence from plasmid DNA in a one-pot procedure. Briefly, one strand of the plasmid DNA is degraded using a nicking ...
    Expression and Purification of Functionally Active Serotonin 5-HT2A Receptor in Insect Cells Using Low-titer Viral Stock
    低滴度病毒载体在昆虫细胞中表达和纯化功能性5-HT2A受体
    作者:Sukanya Mozumder, Gopa Mahesh, Krishnamoorthi Srinivasan, Jayati Sengupta and Sujoy Mukherjee日期:08/05/2020,浏览量:679,Q&A: 0
    [Abstract] The serotonin 5-HT2A receptor (5-HT2AR) is a member of the GPCR family that is important for various neurological functions and whose dysregulation causes many mental health disorders. Structural investigations of 5-HT2AR require the production of functionally active receptors expressed from eukaryotic cell ...