Protocols shared by authors via RaP service
Journal
Determination of copper concentration in rice by ICP-OES
Authors: Yi Li and Shengze Yao
Date: Jul 21, 2024，Views：50
Original protocol used in research article：
The key micronutrient copper orchestrates broad-spectrum virus resistance in rice
Science Advances, Jul 1, 2022; DOI: 10.1126/sciadv.abm0660
Neuronal cultures
Authors: Guiping Wang, Marc Tessier-Lavigne and Xiaowei Zhuang
Date: Jul 17, 2024，Views：37
Original protocol used in research article：
Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration
eLife, May 1, 2019; DOI: 10.7554/eLife.38730
Prediction of functional impact of variants using PROVEAN
Authors: Stefano G Giulieri, Timothy P Stinear and Benjamin P Howden
Date: Jul 14, 2024，Views：31
Original protocol used in research article：
Niche-specific genome degradation and convergent evolution shaping Staphylococcus aureus adaptation during severe infections
eLife, ; DOI: 10.7554/eLife.77195
Sample preparation for FLAG pull down
Authors: Donghai Wang, Hudan Liu, Kaiwei Liang and Guoliang Qing
Date: Jul 10, 2024，Views：59
Original protocol used in research article：
The super elongation complex drives transcriptional addiction in MYCN-amplified neuroblastoma
Science Advances, Mar 29, 2023; DOI: 10.1126/sciadv.adf0005
Tonoplast membrane preparation
Authors: Upendo Lupanga and Karin Schumacher
Date: Jul 9, 2024，Views：29
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
Mouse B cell culture
Authors: Roberta Buono and David A. Fruman
Date: Jul 1, 2024，Views：63
Original protocol used in research article：
Reduced eIF4E function impairs B-cell leukemia without altering normal B-lymphocyte function
iScience, Jun 17, 2021; DOI: 10.1016/j.isci.2021.102748
How to make anti-IgA beads for serum IgA purification
Authors: Yoshihito Nihei and Daisuke Kitamura
Date: Jun 25, 2024，Views：67
Original protocol used in research article：
Identification of IgA autoantibodies targeting mesangial cells redefines the pathogenesis of IgA nephropathy
Science Advances, Mar 22, 2023; DOI: 10.1126/sciadv.add6734
RiboPuromycylation
Authors: Sangeetha Devi Kumar and Sandeep M Eswarappa
Date: Jun 22, 2024，Views：43
Original protocol used in research article：
Evidence for low-level translation in human erythrocytes
Molecular Biology of the Cell, Sep 22, 2022; DOI: 10.1091/mbc.E21-09-0437
RNA pull-down assay
Authors: Chuannan Fan and Peter ten Dijke
Date: Jun 13, 2024，Views：4
Original protocol used in research article：
The lncRNA LETS1 promotes TGF-β–induced EMT and cancer cell migration by transcriptionally activating a TβR1-stabilizing mechanism
SCIENCE SIGNALING, Jun 20, 2023; DOI: 10.1126/scisignal.adf1947
Intracytoplasmic sperm injection（ICSI）
Authors: Yongliang Shang, Fei Gao, Xuejiang Guo, Qing-Yuan Sun, Yunxia Cao and Wei Li
Date: Jun 7, 2024，Views：333
Original protocol used in research article：
Essential role for SUN5 in anchoring sperm head to the tail
eLife, Sep 25, 2017; DOI: 10.7554/eLife.28199
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