Bio-101 - Improve Research Reproducibility

IBS severity scoring system (IBS-SSS)

Authors: Laura Prospero, Giuseppe Riezzo and Francesco Russo
Date: Jun 20, 2022，Views：1384

Original protocol used in research article：

Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity
BMC Gastroenterology, May 22, 2021; DOI: 10.1186/s12876-021-01820-7

Image processing and feature extraction

Author: Anne E Carpenter
Date: Jun 20, 2022，Views：582

Original protocol used in research article：

Systematic morphological profiling of human gene and allele function via Cell Painting
eLife, Mar 18, 2017; DOI: 10.7554/eLife.24060

Cell culture, RNAi experiments, and adenoviral infection

Authors: Zhisheng Xu and Zhenji Gan
Date: Jun 20, 2022，Views：549

Original protocol used in research article：

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength
Nature Communications, Feb 16, 2022; DOI: 10.1038/s41467-022-28557-5

OPP-click

Authors: Madeline Cassani and Geraldine SEYDOUX
Date: Jun 18, 2022，Views：601

Original protocol used in research article：

Puromycin reactivity does not accurately localize translation at the subcellular level
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60303

Immunofluorescence and analyses of PB number and ER-PB colocalization

Authors: Jason E. Lee and Gia K. Voeltz
Date: Jun 18, 2022，Views：570

Original protocol used in research article：

Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI: 10.1126/science.aay7108

In vivo cell depletion

Authors: Michiyuki Matsuda and Hiroshi Ichise
Date: Jun 18, 2022，Views：565

Original protocol used in research article：

Functional visualization of NK cell-mediated killing of metastatic single tumor cells
eLife, Feb 3, 2022; DOI: 10.7554/eLife.76269

Detection of SARS-CoV-2 specific IgG and IgM antibodies

Authors: Ling Wang and Zhi Q. Yao
Date: Jun 17, 2022，Views：540

Original protocol used in research article：

Blockade of SARS-CoV-2 spike protein-mediated cell–cell fusion using COVID-19 convalescent plasma
Scientific Reports, Mar 10, 2021; DOI: 10.1038/s41598-021-84840-3

A2AR expression, purification, and nanodisc reconstitution

Authors: Shuya Kate Huang and R Scott Prosser
Date: Jun 15, 2022，Views：667

Original protocol used in research article：

Allosteric modulation of the adenosine A_2A receptor by cholesterol
eLife, Jan 5, 2022; DOI: 10.7554/eLife.73901

Establishing Endometrial Assembloids to Model Human Embryo Implantation in vitro

Authors: Thomas M Rawlings, Maria Tryfonos, Komal Makwana, Deborah M Taylor, Jan J Brosens and Emma S Lucas
Date: Jun 15, 2022，Views：841

Original protocol used in research article：

Modelling the impact of decidual senescence on embryo implantation in human endometrial assembloids
eLife, Sep 6, 2021; DOI: 10.7554/eLife.69603

The Therapeutic Factors Inventory-19 (TFI-19)

Authors: Nicholas K. Canby and Kristina Eichel
Date: Jun 14, 2022，Views：679

Original protocol used in research article：

The Contribution of Common and Specific Therapeutic Factors to Mindfulness-Based Intervention Outcomes
Frontiers in Psychology, Jan 14, 2021; DOI: 10.3389/fpsyg.2020.603394

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