Bio-101 - Improve Research Reproducibility

Thioflavin S staining

Authors: Zengqiang Yuan, Rui-Yuan Pan and Zengqiang Yuan
Date: Jul 1, 2022，Views：679

Original protocol used in research article：

Sodium rutin ameliorates Alzheimer’s disease–like pathology by enhancing microglial amyloid-β clearance
Science Advances, Feb 27, 2019; DOI: 10.1126/sciadv.aau6328

Cell culture and transfection

Authors: Frank Buchholz and Li Ding
Date: Jul 1, 2022，Views：1107

Original protocol used in research article：

DNA methylation–independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteins
Life Science Alliance, Mar 14, 2022; DOI: 10.26508/lsa.202101321

Oxygen-glucose deprivation (OGD)

Authors: Miren Revuelta and Thomas Schmitz
Date: Jun 30, 2022，Views：747

Original protocol used in research article：

In vitro P38MAPK inhibition in aged astrocytes decreases reactive astrocytes, inflammation and increases nutritive capacity after oxygen-glucose deprivation
Aging (Albany NY), Feb 9, 2021; DOI: 10.18632/aging.202651

Total RNA extraction from fish fin ray

Author: Mari Sato
Date: Jun 29, 2022，Views：602

Original protocol used in research article：

Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing
Scientific Reports, May 13, 2021; DOI: 10.1038/s41598-021-89672-9

Phylogenetic distance analysis and clustering

Author: Ricardo A. Segovia
Date: Jun 29, 2022，Views：587

Original protocol used in research article：

Freezing and water availability structure the evolutionary diversity of trees across the Americas
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aaz5373

Cell proliferation assay

Authors: Linhong Deng, Allen P. Liu and Mingzhi Luo
Date: Jun 29, 2022，Views：593

Original protocol used in research article：

Compression enhances invasive phenotype and matrix degradation of breast Cancer cells via Piezo1 activation
BMC Molecular and Cell Biology, Jan 3, 2022; DOI: 10.1186/s12860-021-00401-6

Study design, inclusion and exclusion criteria.

Authors: Riccardo Pofi, Elisa Giannetta, Daniele Gianfrilli and Andrea M. Isidori
Date: Jun 29, 2022，Views：556

Original protocol used in research article：

Sex-specific effects of daily tadalafil on diabetic heart kinetics in RECOGITO, a randomized, double-blind, placebo-controlled trial
Science Translational Medicine, Jun 15, 2022; DOI: 10.1126/scitranslmed.abl8503

IVDi culture

Authors: Takashi Yoshino and Katsuhiko Hayashi
Date: Jun 28, 2022，Views：619

Original protocol used in research article：

Generation of ovarian follicles from mouse pluripotent stem cells
Science, Jul 16, 2021; DOI: 10.1126/science.abe0237

Study design

Authors: Li-Huei Tsai and Ravikiran M Raju
Date: Jun 28, 2022，Views：574

Original protocol used in research article：

MEF2 is a key regulator of cognitive potential and confers resilience to neurodegeneration
Science Translational Medicine, Nov 3, 2021; DOI: 10.1126/scitranslmed.abd7695

Immunostaining larval body wall neurons in Drosophila

Authors: Jacob S Jaszczak, Laura Devault, Lily Yeh Jan and Yuh Nung Jan
Date: Jun 28, 2022，Views：602

Original protocol used in research article：

Steroid hormone signaling activates thermal nociception during Drosophila peripheral nervous system development
eLife, Mar 30, 2022; DOI: 10.7554/eLife.76464

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