Protocols shared by authors via RaP service
Journal
Spectral decomposition
Author: Magnus Andersson
Date: May 21, 2022，Views：578
Original protocol used in research article：
Tracking Ca2+ ATPase intermediates in real time by x-ray solution scattering
Science Advances, Mar 19, 2020; DOI: 10.1126/sciadv.aaz0981
Purification of IgG specific to full-length VAR2CSA ectodomains
Authors: Justin Y. A. Doritchamou and Patrick E Duffy
Date: May 18, 2022，Views：700
Original protocol used in research article：
A single full-length VAR2CSA ectodomain variant purifies broadly neutralizing antibodies against placental malaria isolates
eLife, Feb 1, 2022; DOI: 10.7554/eLife.76264
Plants, growth conditions, and field plantations
Authors: Danny Kessler and Ian T Baldwin
Date: May 17, 2022，Views：650
Original protocol used in research article：
How scent and nectar influence floral antagonists and mutualists
eLife, Jul 1, 2015; DOI: 10.7554/eLife.07641
AIF subcellular localization analysis and determination of AIF pathology score
Authors: Stephen P. Chelko and Nazareno Paolocci
Date: May 16, 2022，Views：586
Original protocol used in research article：
Exercise triggers CAPN1-mediated AIF truncation inducing myocyte cell death in arrhythmogenic cardiomyopathy
Science Translational Medicine, Feb 16, 2021; DOI: 10.1126/scitranslmed.abf0891
Membrane stability index (MSI)
Author: Natalie De Falco
Date: May 16, 2022，Views：710
Original protocol used in research article：
Geodiversity impacts plant community structure in a semi-arid region
Scientific Reports, Jul 27, 2021; DOI: 10.1038/s41598-021-94698-0
Thermal seizure induction
Author: Daniel K Mulkey
Date: May 13, 2022，Views：605
Original protocol used in research article：
Disordered breathing in a mouse model of Dravet syndrome
eLife, Apr 26, 2019; DOI: 10.7554/eLife.43387
Single-cell isolations
Author: Jonathan Kipnis
Date: May 11, 2022，Views：667
Original protocol used in research article：
Skull and vertebral bone marrow are myeloid cell reservoirs for the meninges and CNS parenchyma
Science, Jul 23, 2021; DOI: 10.1126/science.abf7844
Study design
Authors: Christian Schwartz and Padraic G. Fallon
Date: May 10, 2022，Views：573
Original protocol used in research article：
Innate PD-L1 limits T cell–mediated adipose tissue inflammation and ameliorates diet-induced obesity
Science Translational Medicine, Mar 9, 2022; DOI: 10.1126/scitranslmed.abj6879
NPTX2 ELISA assay
Authors: Paul F Worley and Mei-Fang Xiao
Date: May 10, 2022，Views：638
Original protocol used in research article：
NPTX2 and cognitive dysfunction in Alzheimer’s Disease
eLife, Mar 23, 2017; DOI: 10.7554/eLife.23798
Strains, media, and growth conditions
Authors: Huomiao Ran and Wen-Bing Yin
Date: May 10, 2022，Views：616
Original protocol used in research article：
Fungal-fungal cocultivation leads to widespread secondary metabolite alteration requiring the partial loss-of-function VeA1 protein
Science Advances, Apr 27, 2022; DOI: 10.1126/sciadv.abo6094
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