Protocols shared by authors via RaP service
Journal
RNA isolation, RT-PCR, and library preparation
Authors: Alex Stemm-Wolf and Chad G. Pearson
Date: Jul 19, 2022，Views：710
Original protocol used in research article：
The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
Molecular Biology of the Cell, Sep 9, 2021; DOI: 10.1091/mbc.E21-06-0305
4.4. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Determination by Broth Microdilution and Resazurin Colorimetric Assay
Authors: Oranee Srichaiyapol and Rina Patramanon
Date: Jul 18, 2022，Views：921
Original protocol used in research article：
Tannic Acid-Stabilized Silver Nanoparticles Used in Biomedical Application as an Effective Antimelioidosis and Prolonged Efflux Pump Inhibitor against Melioidosis Causative Pathogen
Molecules, Feb 14, 2021; DOI: 10.3390/molecules26041004
Novel environment test and novel object test
Author: Allyson F. Ipema
Date: Jul 18, 2022，Views：649
Original protocol used in research article：
Providing live black soldier fly larvae (Hermetia illucens) improves welfare while maintaining performance of piglets post-weaning
Scientific Reports, Apr 1, 2021; DOI: 10.1038/s41598-021-86765-3
Flow cytometry of PBMCs
Authors: Heather M. Gibson, Justin B. Hackett and Claire McCarthy-Leo
Date: Jul 18, 2022，Views：653
Original protocol used in research article：
A diversity outbred F1 mouse model identifies host-intrinsic genetic regulators of response to immune checkpoint inhibitors
Oncoimmunology, Apr 20, 2022; DOI: 10.1080/2162402X.2022.2064958
2.5.4. Cupric Ion Reducing Antioxidant Capacity (CUPRAC) Assay
Authors: Ahmed G. Darwish, Protiva Rani Das and Islam El-Sharkawy
Date: Jul 17, 2022，Views：690
Original protocol used in research article：
Untargeted Metabolomics and Antioxidant Capacities of Muscadine Grape Genotypes during Berry Development
Antioxidants, Jun 4, 2021; DOI: 10.3390/antiox10060914
Untargeted and targeted lipidomics – statistical analysis: Factoria ANOVA in R
Authors: Lucas Carminatti Pantaleao, Isabella Inzani and Susan E Ozanne
Date: Jul 15, 2022，Views：731
Original protocol used in research article：
Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner
eLife, Jan 13, 2022; DOI: 10.7554/eLife.69078
preparation of nanoparticles by sonication
Authors: Zhao Zhang, Vincent Terrasson and Erwann Guénin
Date: Jul 15, 2022，Views：606
Original protocol used in research article：
Lignin Nanoparticles and Their Nanocomposites
Nanomaterials, May 19, 2021; DOI: 10.3390/nano11051336
Preparation of cell extracts
Authors: Toshiaki Tanaka and Koji Moriya
Date: Jul 15, 2022，Views：627
Original protocol used in research article：
Visualized procollagen Iα1 demonstrates the intracellular processing of propeptides
Life Science Alliance, Feb 18, 2022; DOI: 10.26508/lsa.202101060
Reverse transcription PCR and quantitative PCR analysis
Author: Sung Wook Seo
Date: Jul 15, 2022，Views：709
Original protocol used in research article：
GNAQ knockdown promotes bone metastasis through epithelial-mesenchymal transition in lung cancer cells
Bone & Joint Research, May 17, 2021; DOI: 10.1302/2046-3758.105.BJR-2020-0262.R3
Dissociation of adult rodent DRG for single-cell RNAseq
Authors: Oshri Avraham and Valeria Cavalli
Date: Jul 14, 2022，Views：897
Original protocol used in research article：
Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
eLife, Sep 29, 2021; DOI: 10.7554/eLife.68457
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