2.5.4. Cupric Ion Reducing Antioxidant Capacity (CUPRAC) Assay

Cupric Reducing Antioxidant Power (CUPRAC Assay)

The cupric ion reducing antioxidant capacity of the extracts was determined according to the method of Apak et al. (2008). 100 μL of sample extract was mixed with 1 mL each of 10 mM of cupper chloride solution, 7.5 mM of neocuproine alcoholic solution (99.9% ethanol), 1 M (pH 7.0) of ammonium acetate buffer solution, and 1 mL of distilled water to make final volume 4.1 mL. After 30 min, the absorbance was recorded at 450 nm against the reagent blank. Standard curve was prepared using different concentrations of Trolox. The results were expressed as μmol Trolox/g.

100 μL of sample extract.

1 mL 10 mM of cupper chloride solution.

1mL 7.5 mM of neocuproine alcoholic solution (99.9% ethanol).

1mL 1 M (pH 7.0) of ammonium acetate buffer solution.

1 mL of distilled water to make final volume 4.1 ml.