Protocols shared by authors via RaP service
Journal
Single-cell photoablation of MN2
Author: Kohji Hotta
Date: Jun 8, 2022，Views：610
Original protocol used in research article：
A single motor neuron determines the rhythm of early motor behavior in Ciona
Science Advances, Dec 10, 2021; DOI: 10.1126/sciadv.abl6053
In vitro experiments
Authors: Mark D. Bunting, Maulik Vyas and Shadmehr Demehri
Date: Jun 8, 2022，Views：598
Original protocol used in research article：
Extracellular matrix proteins regulate NK cell function in peripheral tissues
Science Advances, Mar 16, 2022; DOI: 10.1126/sciadv.abk3327
Comparison of mutation severity, gene intolerance, and mutation distribution
Authors: Guan Ning Lin, Weichen Song and Zhen Wang
Date: Jun 7, 2022，Views：593
Original protocol used in research article：
De novo mutations identified by whole-genome sequencing implicate chromatin modifications in obsessive-compulsive disorder
Science Advances, Jan 12, 2022; DOI: 10.1126/sciadv.abi6180
Permutation test
Authors: Guan Ning Lin, Weichen Song and Zhen Wang
Date: Jun 7, 2022，Views：565
Original protocol used in research article：
De novo mutations identified by whole-genome sequencing implicate chromatin modifications in obsessive-compulsive disorder
Science Advances, Jan 12, 2022; DOI: 10.1126/sciadv.abi6180
Total RNA-seq
Authors: Ane Martin Anduaga and Sebastian Kadener
Date: Jun 6, 2022，Views：570
Original protocol used in research article：
Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila
eLife, Nov 8, 2019; DOI: 10.7554/eLife.44642
Drosophila S2 cells RNA extraction
Authors: Subhendu Roy Choudhury and Saverio Brogna
Date: Jun 5, 2022，Views：599
Original protocol used in research article：
Exon junction complex proteins bind nascent transcripts independently of pre-mRNA splicing in Drosophila melanogaster
eLife, Nov 23, 2016; DOI: 10.7554/eLife.19881
Translation efficiency calculation
Authors: David H Burkhardt, Silvi Rouskin, Gene-Wei Li, Jonathan S Weissman, Carol A Gross and Yan Zhang
Date: Jun 3, 2022，Views：804
Original protocol used in research article：
Operon mRNAs are organized into ORF-centric structures that predict translation efficiency
eLife, Jan 31, 2017; DOI: 10.7554/eLife.22037
Immunofluorescence
Authors: Ying Ying Yiu, Grace Blacker and Irving L. Weissman
Date: Jun 2, 2022，Views：594
Original protocol used in research article：
CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone
J Immunol, Mar 15, 2022; DOI: 10.4049/jimmunol.2100352
ERCC spike-in normalized mRNA-seq for cultured cells (human)
Authors: Mark W. Zimmerman, Brian J. Abraham and A. Thomas Look
Date: Jun 1, 2022，Views：577
Original protocol used in research article：
Retinoic acid rewires the adrenergic core regulatory circuitry of childhood neuroblastoma
Science Advances, Oct 20, 2021; DOI: 10.1126/sciadv.abe0834
Western blot assay
Authors: Chenkui Miao and Li Jia
Date: May 30, 2022，Views：639
Original protocol used in research article：
RB1 loss overrides PARP inhibitor sensitivity driven by RNASEH2B loss in prostate cancer
Science Advances, Feb 18, 2022; DOI: 10.1126/sciadv.abl9794
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