Bio-101 - Improve Research Reproducibility

preparation of nanoparticles by sonication

Authors: Zhao Zhang, Vincent Terrasson and Erwann Guénin
Date: Jul 15, 2022，Views：553

Original protocol used in research article：

Lignin Nanoparticles and Their Nanocomposites
Nanomaterials, May 19, 2021; DOI: 10.3390/nano11051336

Preparation of cell extracts

Authors: Toshiaki Tanaka and Koji Moriya
Date: Jul 15, 2022，Views：570

Original protocol used in research article：

Visualized procollagen Iα1 demonstrates the intracellular processing of propeptides
Life Science Alliance, Feb 18, 2022; DOI: 10.26508/lsa.202101060

Reverse transcription PCR and quantitative PCR analysis

Author: Sung Wook Seo
Date: Jul 15, 2022，Views：611

Original protocol used in research article：

GNAQ knockdown promotes bone metastasis through epithelial-mesenchymal transition in lung cancer cells
Bone & Joint Research, May 17, 2021; DOI: 10.1302/2046-3758.105.BJR-2020-0262.R3

Dissociation of adult rodent DRG for single-cell RNAseq

Authors: Oshri Avraham and Valeria Cavalli
Date: Jul 14, 2022，Views：689

Original protocol used in research article：

Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
eLife, Sep 29, 2021; DOI: 10.7554/eLife.68457

Protein extraction and ELISA

Author: Idit Sagiv Barfi
Date: Jul 13, 2022，Views：668

Original protocol used in research article：

Intratumoral immunotherapy relies on B and T cell collaboration
Science Immunology, May 27, 2022; DOI: 10.1126/sciimmunol.abn5859

HLA Typing by High-Resolution Next-Generation Sequencing

Authors: Roos A. W. Wennink and Jonas J. W. Kuiper
Date: Jul 13, 2022，Views：588

Original protocol used in research article：

Next-Generation HLA Sequence Analysis Uncovers Shared Risk Alleles Between Clinically Distinct Forms of Childhood Uveitis
Investigative Ophthalmology & Visual Science, Jul 13, 2021; DOI: 10.1167/iovs.62.9.19

Cell-to-cell fusion assay

Authors: Tarun Mishra, Rishikesh Dalavi and Ajit Chande
Date: Jul 13, 2022，Views：1227

Original protocol used in research article：

SARS-CoV-2 spike E156G/Δ157-158 mutations contribute to increased infectivity and immune escape
Life Science Alliance, Mar 16, 2022; DOI: 10.26508/lsa.202201415

ChR2-assisted circuit mapping

Author: Ji Liu
Date: Jul 13, 2022，Views：589

Original protocol used in research article：

Glucose-sensing glucagon-like peptide-1 receptor neurons in the dorsomedial hypothalamus regulate glucose metabolism
Science Advances, Jun 8, 2022; DOI: 10.1126/sciadv.abn5345

Isolation of mouse footpad cells for immunophenotyping

Authors: Anthony Torres-Ruesta, Laurent Renia and Lisa FP Ng
Date: Jul 12, 2022，Views：657

Original protocol used in research article：

Malaria abrogates O’nyong–nyong virus pathologies by restricting virus infection in nonimmune cells
Life Science Alliance, Jan 17, 2022; DOI: 10.26508/lsa.202101272

Ex vivo organotypic cerebellum slice culture (OCSC)

Authors: Charles Capdeville and Martin Baumgartner
Date: Jul 12, 2022，Views：599

Original protocol used in research article：

Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
Life Science Alliance, Mar 16, 2022; DOI: 10.26508/lsa.202201380

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