Bio-101 - Improve Research Reproducibility

Allelic exchange constructs

Authors: Eva S Istvan, Akhil Vaidya and Daniel E Goldberg
Date: Jul 6, 2022，Views：537

Original protocol used in research article：

Plasmodium Niemann-Pick type C1-related protein is a druggable target required for parasite membrane homeostasis
eLife, Mar 19, 2019; DOI: 10.7554/eLife.40529

Image acquisition and analysis

Authors: Ana C. Laly and John T. Connelly
Date: Jul 6, 2022，Views：540

Original protocol used in research article：

The keratin network of intermediate filaments regulates keratinocyte rigidity sensing and nuclear mechanotransduction
Science Advances, Jan 27, 2021; DOI: 10.1126/sciadv.abd6187

Tumor processing for CAF isolation

Authors: Yuki Miyai and Atsushi Enomoto
Date: Jul 5, 2022，Views：647

Original protocol used in research article：

Meflin-positive cancer-associated fibroblasts enhance tumor response to immune checkpoint blockade
Life Science Alliance, Mar 1, 2022; DOI: 10.26508/lsa.202101230

In vivo muscle regeneration and cross-sectional immunofluorescence

Authors: Claudia Dominici and Stéphane Richard
Date: Jul 5, 2022，Views：546

Original protocol used in research article：

Muscle stem cell polarity requires QKI-mediated alternative splicing of Integrin Alpha-7 (Itga7)
Life Science Alliance, Feb 14, 2022; DOI: 10.26508/lsa.202101192

Cell suspension preparation for flow cytometry and flow cytometry analysis

Authors: Nehemiah Cox and Frederic Geissmann
Date: Jul 5, 2022，Views：557

Original protocol used in research article：

Diet-regulated production of PDGFcc by macrophages controls energy storage
Science, Jul 2, 2021; DOI: 10.1126/science.abe9383

Thioflavin S staining

Authors: Zengqiang Yuan, Rui-Yuan Pan and Zengqiang Yuan
Date: Jul 1, 2022，Views：612

Original protocol used in research article：

Sodium rutin ameliorates Alzheimer’s disease–like pathology by enhancing microglial amyloid-β clearance
Science Advances, Feb 27, 2019; DOI: 10.1126/sciadv.aau6328

Cell culture and transfection

Authors: Frank Buchholz and Li Ding
Date: Jul 1, 2022，Views：1084

Original protocol used in research article：

DNA methylation–independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteins
Life Science Alliance, Mar 14, 2022; DOI: 10.26508/lsa.202101321

Oxygen-glucose deprivation (OGD)

Authors: Miren Revuelta and Thomas Schmitz
Date: Jun 30, 2022，Views：718

Original protocol used in research article：

In vitro P38MAPK inhibition in aged astrocytes decreases reactive astrocytes, inflammation and increases nutritive capacity after oxygen-glucose deprivation
Aging (Albany NY), Feb 9, 2021; DOI: 10.18632/aging.202651

Total RNA extraction from fish fin ray

Author: Mari Sato
Date: Jun 29, 2022，Views：584

Original protocol used in research article：

Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing
Scientific Reports, May 13, 2021; DOI: 10.1038/s41598-021-89672-9

Phylogenetic distance analysis and clustering

Author: Ricardo A. Segovia
Date: Jun 29, 2022，Views：553

Original protocol used in research article：

Freezing and water availability structure the evolutionary diversity of trees across the Americas
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aaz5373

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