Bio-101 - Improve Research Reproducibility

Mitochondrial OXPHOS efficiency and capacity

Authors: Jeffrey E Pessin and Yan Tang
Date: Jun 12, 2022，Views：578

Original protocol used in research article：

TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling
eLife, Mar 7, 2022; DOI: 10.7554/eLife.73360

Lentivirus infection

Author: Edward R Kastenhuber
Date: Jun 9, 2022，Views：665

Original protocol used in research article：

Coagulation factors directly cleave SARS-CoV-2 spike and enhance viral entry
eLife, Mar 16, 2022; DOI: 10.7554/eLife.77444

Lipid content measurement

Authors: Pan Su, Qiang Wang and Qing Yi
Date: Jun 9, 2022，Views：569

Original protocol used in research article：

Enhanced lipid accumulation and metabolism are required for the differentiation and activation of tumor-associated macrophages
Cancer Res, Feb 3, 2020; DOI: 10.1158/0008-5472.CAN-19-2994

2.11. Glucose uptake and lactate production

Authors: Hui Wang and Xiaoxia Liu
Date: Jun 9, 2022，Views：566

Original protocol used in research article：

Enhancing cancer‐associated fibroblast fatty acid catabolism within a metabolically challenging tumor microenvironment drives colon cancer peritoneal metastasis
Molecular Oncology, Feb 16, 2021; DOI: 10.1002/1878-0261.12917

Negative staining of HIV viral particles permeabilised with PFO with capsid stabilised with IP6

Authors: Derrick Lau, Chantal L Márquez, Michael Parker and Till Böcking
Date: Jun 8, 2022，Views：722

Original protocol used in research article：

Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis
eLife, Jun 7, 2018; DOI: 10.7554/eLife.34772

Single-cell photoablation of MN2

Author: Kohji Hotta
Date: Jun 8, 2022，Views：645

Original protocol used in research article：

A single motor neuron determines the rhythm of early motor behavior in Ciona
Science Advances, Dec 10, 2021; DOI: 10.1126/sciadv.abl6053

In vitro experiments

Authors: Mark D. Bunting, Maulik Vyas and Shadmehr Demehri
Date: Jun 8, 2022，Views：619

Original protocol used in research article：

Extracellular matrix proteins regulate NK cell function in peripheral tissues
Science Advances, Mar 16, 2022; DOI: 10.1126/sciadv.abk3327

Comparison of mutation severity, gene intolerance, and mutation distribution

Authors: Guan Ning Lin, Weichen Song and Zhen Wang
Date: Jun 7, 2022，Views：611

Original protocol used in research article：

De novo mutations identified by whole-genome sequencing implicate chromatin modifications in obsessive-compulsive disorder
Science Advances, Jan 12, 2022; DOI: 10.1126/sciadv.abi6180

Permutation test

Authors: Guan Ning Lin, Weichen Song and Zhen Wang
Date: Jun 7, 2022，Views：594

Original protocol used in research article：

De novo mutations identified by whole-genome sequencing implicate chromatin modifications in obsessive-compulsive disorder
Science Advances, Jan 12, 2022; DOI: 10.1126/sciadv.abi6180

Total RNA-seq

Authors: Ane Martin Anduaga and Sebastian Kadener
Date: Jun 6, 2022，Views：631

Original protocol used in research article：

Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila
eLife, Nov 8, 2019; DOI: 10.7554/eLife.44642

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