First of all, thank you for your interest in our research paper.
Optical fiber was implanted into the DMV (AP: −3.6 mm from the lambda; ML: 0 mm from the midline; DV:−3.5 mm from dura) . The length of the optical fiber was 5.0 mm from the bottom of the ceramic ferrule.
Hope the above answers are helpful to you.
Zhaohuan, Huang
School of Information Science and Technology, USTC.
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How to cite:
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
Huang, Z., Liu, L., Zhang, J., Conde, K., Phansalkar, J., Li, Z., Yao, L., Xu, Z., Wang, W., Zhou, J., Bi, G., Wu, F., Seeley, R. J., Scott, M. M., Zhan, C., Pang, Z. P. and Liu, J.(2022). Glucose-sensing glucagon-like peptide-1 receptor neurons in the dorsomedial hypothalamus regulate glucose metabolism. Science Advances 8(23). DOI: 10.1126/sciadv.abn5345
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