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ChR2-assisted circuit mapping

JL Ji Liu

Last updated date: Jul 13, 2022 Views: 576 Forks: 0

original An abbreviated version of this protocol was published in Science Advances
Glucose-sensing glucagon-like peptide-1 receptor neurons in the dorsomedial hypothalamus regulate glucose metabolism
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Hello,

First of all, thank you for your interest in our research paper. 

Optical fiber was implanted into the DMV (AP: −3.6 mm from the lambda; ML: 0 mm from the midline; DV:−3.5 mm from dura) . The length of the optical fiber was 5.0 mm from the bottom of the ceramic ferrule.

Hope the above answers are helpful to you.

 

Zhaohuan, Huang

School of Information Science and Technology, USTC.
 

 

 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Liu, J(2022). ChR2-assisted circuit mapping. Bio-protocol Preprint. bio-protocol.org/prep1791.
  2. Huang, Z., Liu, L., Zhang, J., Conde, K., Phansalkar, J., Li, Z., Yao, L., Xu, Z., Wang, W., Zhou, J., Bi, G., Wu, F., Seeley, R. J., Scott, M. M., Zhan, C., Pang, Z. P. and Liu, J.(2022). Glucose-sensing glucagon-like peptide-1 receptor neurons in the dorsomedial hypothalamus regulate glucose metabolism. Science Advances 8(23). DOI: 10.1126/sciadv.abn5345

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