Bio-101 - Improve Research Reproducibility

Drosophila S2 cells RNA extraction

Authors: Subhendu Roy Choudhury and Saverio Brogna
Date: Jun 5, 2022，Views：672

Original protocol used in research article：

Exon junction complex proteins bind nascent transcripts independently of pre-mRNA splicing in Drosophila melanogaster
eLife, Nov 23, 2016; DOI: 10.7554/eLife.19881

Translation efficiency calculation

Authors: David H Burkhardt, Silvi Rouskin, Gene-Wei Li, Jonathan S Weissman, Carol A Gross and Yan Zhang
Date: Jun 3, 2022，Views：1051

Original protocol used in research article：

Operon mRNAs are organized into ORF-centric structures that predict translation efficiency
eLife, Jan 31, 2017; DOI: 10.7554/eLife.22037

Immunofluorescence

Authors: Ying Ying Yiu, Grace Blacker and Irving L. Weissman
Date: Jun 2, 2022，Views：628

Original protocol used in research article：

CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone
J Immunol, Mar 15, 2022; DOI: 10.4049/jimmunol.2100352

ERCC spike-in normalized mRNA-seq for cultured cells (human)

Authors: Mark W. Zimmerman, Brian J. Abraham and A. Thomas Look
Date: Jun 1, 2022，Views：720

Original protocol used in research article：

Retinoic acid rewires the adrenergic core regulatory circuitry of childhood neuroblastoma
Science Advances, Oct 20, 2021; DOI: 10.1126/sciadv.abe0834

Western blot assay

Authors: Chenkui Miao and Li Jia
Date: May 30, 2022，Views：663

Original protocol used in research article：

RB1 loss overrides PARP inhibitor sensitivity driven by RNASEH2B loss in prostate cancer
Science Advances, Feb 18, 2022; DOI: 10.1126/sciadv.abl9794

Test for tannins (ferric chloride test)

Authors: Dereje Nigussie and Dereje Nigussie
Date: May 30, 2022，Views：3789

Original protocol used in research article：

Antibacterial activity of methanol extracts of the leaves of three medicinal plants against selected bacteria isolated from wounds of lymphoedema patients
BMC Complementary Medicine and Therapies, Jan 3, 2021; DOI: 10.1186/s12906-020-03183-0

10x genomics

Authors: Sudhir Gopal Tattikota and Norbert Perrimon
Date: May 27, 2022，Views：681

Original protocol used in research article：

A single-cell survey of Drosophila blood
eLife, May 12, 2020; DOI: 10.7554/eLife.54818

Transfections of poly(I:C), DNA, miRNA mimics and Antagomirs

Authors: Jeroen Witteveldt and Sara Macias
Date: May 27, 2022，Views：2515

Original protocol used in research article：

MicroRNA-deficient mouse embryonic stem cells acquire a functional interferon response
eLife, Apr 23, 2019; DOI: 10.7554/eLife.44171

SOD Activity

Authors: Swetha Sudha Nagamalla and Someswar Rao Sagurthi
Date: May 26, 2022，Views：1067

Original protocol used in research article：

Morpho-Physiological and Proteomic Response of Bt-Cotton and Non-Bt Cotton to Drought Stress
Frontiers in Plant Science, May 10, 2021; DOI: 10.3389/fpls.2021.663576

Generation of Genotypes

Authors: Jose Alquicira-Hernandez, Kristof Wing, Alex W. Hewitt and Joseph E. Powell
Date: May 26, 2022，Views：707

Original protocol used in research article：

Single-cell eQTL mapping identifies cell type–specific genetic control of autoimmune disease
Science, Apr 8, 2022; DOI: 10.1126/science.abf3041

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