Protocols shared by authors via RaP service
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Authors: Aurélien Marc and Jeremie Guedj
Date: May 5, 2022，Views：609
Original protocol used in research article：
Quantifying the relationship between SARS-CoV-2 viral load and infectiousness
eLife, Sep 27, 2021; DOI: 10.7554/eLife.69302
VLP sequencing
Author: Kate L. Jeffrey
Date: May 4, 2022，Views：615
Original protocol used in research article：
Human enteric viruses autonomously shape inflammatory bowel disease phenotype through divergent innate immunomodulation
Science Immunology, Apr 8, 2022; DOI: 10.1126/sciimmunol.abn6660
ATAC-seq methodology used in Dong et al., eLIFE, 2020
Authors: Xiaoyun Xing, Chen Dong, Thorold W Theunissen and Ting Wang
Date: May 3, 2022，Views：668
Original protocol used in research article：
Derivation of trophoblast stem cells from naïve human pluripotent stem cells
eLife, Feb 12, 2020; DOI: 10.7554/eLife.52504
Construction of the FICA system
Authors: Meiyan Wang and Haifeng Ye
Date: May 3, 2022，Views：612
Original protocol used in research article：
A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation
Science Advances, Dec 10, 2021; DOI: 10.1126/sciadv.abh2358
Immunohistochemistry of murine retina
Authors: Mervin C. Yoder, Maria B. Grant and Sergio Li Calzi
Date: May 2, 2022，Views：619
Original protocol used in research article：
Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice
Science Advances, Mar 4, 2022; DOI: 10.1126/sciadv.abm5559
Dot blot assay
Authors: Yong-gui Wu and Xiao-ming Meng
Date: Apr 29, 2022，Views：778
Original protocol used in research article：
Inhibition of METTL3 attenuates renal injury and inflammation by alleviating TAB3 m6A modifications via IGF2BP2-dependent mechanisms
Science Translational Medicine, Apr 13, 2022; DOI: 10.1126/scitranslmed.abk2709
IMC data
Authors: James N Arnold and Shahram Kordasti
Date: Apr 28, 2022，Views：628
Original protocol used in research article：
ImmunoCluster provides a computational framework for the nonspecialist to profile high-dimensional cytometry data
eLife, Apr 30, 2021; DOI: 10.7554/eLife.62915
Isolation and cryopreservation of gonads
Authors: Michael J McGrew and Jun Hu
Date: Apr 28, 2022，Views：617
Original protocol used in research article：
A low-tech, cost-effective and efficient method for safeguarding genetic diversity by direct cryopreservation of poultry embryonic reproductive cells
eLife, Jan 25, 2022; DOI: 10.7554/eLife.74036
JAGuaR Alignment
Author: Richard Corbett
Date: Apr 26, 2022，Views：579
Original protocol used in research article：
A Scalable Strand-Specific Protocol Enabling Full-Length Total RNA Sequencing From Single Cells
Frontiers in Genetics, Jun 3, 2021; DOI: 10.3389/fgene.2021.665888
Preparation of bovine serum albumin (BSA)-conjugated palmitic acid
Author: Fei Hua
Date: Apr 26, 2022，Views：796
Original protocol used in research article：
Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway
BMC Nephrology, Jun 10, 2021; DOI: 10.1186/s12882-021-02391-1
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